AW: [Histonet] Bcl-2

[Gudrun Lang]

Valerie,
I would look at the fixation time of the tonsils and on their storage time.
If the tonsils are fixed much longer than your lymphoma specimens a harsher
pretreatment is necessary.
If the control-tonsils are stored for long time the surface could suffer
from oxidation. Do you have fresh tonsil for testing?

Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Hannen,
Valerie
Gesendet: Mittwoch, 24. Februar 2010 17:48
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Bcl-2

Hi everyone,
 
 I am hoping you all can help me solve a problem that I am having with
my Bcl-2 stain. I can get it to stain Follicular Lymphoma tissues, but
for the life of me can not
 
 get normal tonsil tissue to stain.  I use a Retrieval solution with a
pH of 8, use Avidin / Biotin solutions, incubate in the primary for 60
minutes. The tonsil tissues that I 
 
am testing are inhouse fixed and processed tissues. We use Zinc Formalin
for our fixation. Now that I think of it, I am also having trouble
getting a purchased control of 
 
Lymphoma tissue to stain.  The kit that we are using is the Histostain +
kit, and we do our Immuno's manually. 
 
 What am I doing wrong or what am I not doing?  
 
Thanks in advance to any and all replies.
 
 
Valerie Hannen, Histotechnologist
Parrish Medical Center
Titusville, Florida
 


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