[Histonet] Bcl-2

[Rene J Buesa]

I always did HIER at pH6 for BCL-2, diluted  at 1:10 (DAKO Ab) during 30 min incubarion. FFPE tonsil as control.
René J.

--- On Wed, 2/24/10, Hannen, Valerie <Valerie.Hannen <@t> parrishmed.com> wrote:


From: Hannen, Valerie <Valerie.Hannen <@t> parrishmed.com>
Subject: [Histonet] Bcl-2
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, February 24, 2010, 11:48 AM


Hi everyone,

I am hoping you all can help me solve a problem that I am having with
my Bcl-2 stain. I can get it to stain Follicular Lymphoma tissues, but
for the life of me can not

get normal tonsil tissue to stain.  I use a Retrieval solution with a
pH of 8, use Avidin / Biotin solutions, incubate in the primary for 60
minutes. The tonsil tissues that I 

am testing are inhouse fixed and processed tissues. We use Zinc Formalin
for our fixation. Now that I think of it, I am also having trouble
getting a purchased control of 

Lymphoma tissue to stain.  The kit that we are using is the Histostain +
kit, and we do our Immuno's manually. 

What am I doing wrong or what am I not doing?  

Thanks in advance to any and all replies.


Valerie Hannen, Histotechnologist
Parrish Medical Center
Titusville, Florida



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