[Histonet] Liver tissue

[V. Neubert]

Longer dehydration, longer paraffination is what I would suggest.

Splintering occured when I used freezing spray and the tissue got too cold.

Try to increase humidity while sectioning (gently breathe on the block
while sectioning). Should help against crumbling.

Good luck, post your dehydration schedule please!


> I am having trouble cutting FFPE liver sections. I have embedded the tissue manually going from fomalin to water to increasing ethanol to xylene and to paraffin. I can get good sections by soaking the block with ice cold water before taking a few sections but then have to re-soak in order to take more sections. If I do not soak the tissue on the chuck the liver crumbles and falls out of the paraffin. The soaking and sectioning is very time consuming. Does anyone have an idea what might be the problem?
>
> Thanks for any help!!
> Mike
>
>
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