[Histonet] Formula 83
Beth Austin
baustin <@t> cbgbiotech.com
Fri Feb 5 13:24:43 CST 2010
Mr. Richmond,
I was contacted off site and asked to clarify something you posted to the
Histonet. I hope you do not mind.
The flashpoint of Formula 83 is not 7 degrees Fahrenheit.
It is 7 degrees Celsius, or 45 degrees Fahrenheit. Also please note that the
auto ignite temperature is 482 degrees Fahrenheit.
Respectfully,
Beth Austin-Sell
CBG Biotech
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Today's Topics:
1. RE: GI, Uro, or Derm Path Lab Set Up (Sally Price)
2. Formula 83 (Robert Richmond)
3. RE: cleaning cryostat (Ingles Claire )
4. What slides do you use? (Scott Hendricksen)
5. New slide drier recommendations please (Scott Hendricksen)
6. Re: BioCare immunostainer (Pat Laurie)
7. Slide baking before IHC (Pat Laurie)
8. Re: RE: GI, Uro, or Derm Path Lab Set Up (Jay Lundgren)
9. Temperature and Humidity Monitoring System
(Teri.Hallada <@t> midmichigan.org)
10. RE: Slide baking before IHC (Morken, Tim)
11. RE: Temperature and Humidity Monitoring System (Liz Chlipala)
----------------------------------------------------------------------
Message: 1
Date: Thu, 4 Feb 2010 19:48:23 -0500
From: Sally Price <sprice2003 <@t> gmail.com>
Subject: [Histonet] RE: GI, Uro, or Derm Path Lab Set Up
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<ffd26fa81002041648y5e648596sc4034bf7e84378e1 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
...and another thing -- since when is it OK to post such blatant
solicitations on the HistoNet? other vendors are chastised for this on a
regular basis!
Sally
------------------------------
Message: 2
Date: Thu, 4 Feb 2010 21:14:50 -0500
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Formula 83
To: "Histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<abea52a61002041814g4e82823dx50ebc41d77dd8667 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Allison Hutton, HTL(ASCP)cm, Lead Tech Histology, Doylestown Hospital,
Doylestown, Pennsylvania asks:
>>For those of you who are using Formula 83, could you please contact me off
line? We have completed our demo but still have a few questions.<<
Formula 83, offered by CBG Biotech Ltd., is a napthenic (cycloalkane)
hydrocarbon used as a xylene substitute. It differs in structure from
the aliphatic hydrocarbons such as Richard Allan's Clear-rite 3. It's
in use in a lab I'm working in at the moment.
I have two reservations about it. The first is that, unlike the
aliphatics, it has a definite odor, which could be objectionable in a
confined space.
The second reservation: Formula 83 has a flash point of only 7 degrees
Fahrenheit, lower than xylene (78 degrees) and other aliphatics, some
of which have flash points as high as 144 F. The fire hazard here is
worth considering.
Formula 83 has been discussed before on Histonet, and the posts are
worth looking up.
When purchasing a new aliphatic, there are several important
considerations. Your purchasing people need to understand that they
can't change the xylene substitute on you, just because something a
bit cheaper comes along. If you recycle, every different xylene
substitute has a different distillation routine. It's important to ask
everyone in the lab - in particular, the cytotechnologist is likely to
have very strong opinions about the solvents they use in their
ascending sequence before coverslipping.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 3
Date: Thu, 4 Feb 2010 22:44:24 -0600
From: "Ingles Claire " <CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] cleaning cryostat
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F2F030053F9B7345831BED293A6D57E109A802 <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
I work in a Mohs Lab. (high output frozen skin) We wipe out the cryostat
with 95% ETOH and clear out the shavings every day we use our machines. We
normally tear down and completely defrost our cryostats every other month or
so, depending on humidity and frost build up. (and available time to have a
cryostat down.) We are not inspected by CAP, but JCAHO and CLIA who are at
least as stringent.
Claire
________________________________
I would like this information also. This is the first year we are CAP so
any help I can get would be appreciated. Thanks so much have a great
Friday and Week end.
Kathy Gorham H.T.
------------------------------
Message: 4
Date: Thu, 4 Feb 2010 23:24:06 -0700
From: Scott Hendricksen <lscott <@t> sfcn.org>
Subject: [Histonet] What slides do you use?
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <E433DACC-DD39-4575-BC66-E249E37FFFE6 <@t> sfcn.org>
Content-Type: text/plain; charset=us-ascii
Hi,
I was wondering what microscope slides you use. For the last
couple of years I have noticed that a lot of the different brand slides are
dirty. We have used Erie brand slides for many years, and they are the best
quality I can find. Now even a lot of the Erie slides are dirty and we end
up throwing a few away. Any suggestions for good clean slides at a
reasonable price?
Thanks again !
Scott Hendricksen HT(ASCP)
------------------------------
Message: 5
Date: Thu, 4 Feb 2010 23:35:33 -0700
From: Scott Hendricksen <lscott <@t> sfcn.org>
Subject: [Histonet] New slide drier recommendations please
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <7A5A7B7C-59A0-4AA1-BDBA-09EAD2C11F75 <@t> sfcn.org>
Content-Type: text/plain; charset=us-ascii
Hi,
Does anyone have a suggestion for a forced air slide drier? We
were looking at possibly the TBS SD-II-120.
We have already tried a slide oven but it takes too long for the slides to
dry, it was lacking a fan to circulate the air.
Thanks,
Scott Hendricksen HT(ASCP)
------------------------------
Message: 6
Date: Thu, 4 Feb 2010 22:47:22 -0800
From: Pat Laurie <foreightl <@t> gmail.com>
Subject: Re: [Histonet] BioCare immunostainer
To: "Paal, Edina E." <Edina.Paal <@t> va.gov>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<bdfbc2371002042247yf9d5a7dm63d0bae7bfca878f <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
We have an intellipath. It is a great machine. They have several unique
innovations which makes customizing the work much easier. It depends what
you need from the machine, but this had many innovations which helped our
IHC department. It is also a completely open system which is becoming more
and more difficult to find nowdays.
On Thu, Feb 4, 2010 at 7:54 AM, Paal, Edina E. <Edina.Paal <@t> va.gov> wrote:
> We are at the verge of replacing our old immunostainer. We saw an
> excellent introduction to BioCare but I would like to hear from real
> people using it. Anything that makes you happy or unhappy? All
> information would be appreciated.
>
>
> Edina Paal, M.D.
> Pathologist
> VA Medical Center, Washington DC
> 50 Irving St, N.W., Rm GB205
> Washington, DC 20422
>
> Tel: (202) 518-4619
> Fax: (202) 745-8284
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie <@t> cellnetix.com
------------------------------
Message: 7
Date: Fri, 5 Feb 2010 00:12:43 -0800
From: Pat Laurie <foreightl <@t> gmail.com>
Subject: [Histonet] Slide baking before IHC
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<bdfbc2371002050012q33426f6ckb0dc07fa6a748990 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Histonet,
I have heard rather anecdotally that if you bake slides at high temps (75
degrees to 80 degrees) before IHC for a long period of time (several hours
to days), you may affect antigenicity for some antibodies. Has there been
any study done about this? Also, what if it is a high temp (around 75
degrees C) for just 20 minutes? Or a low temperature for a long period of
time?
Thanks in advance for your input.
--
Patrick Laurie HT(ASCP)QIHC
plaurie <@t> cellnetix.com
------------------------------
Message: 8
Date: Fri, 5 Feb 2010 06:59:24 -0500
From: Jay Lundgren <jaylundgren <@t> gmail.com>
Subject: Re: [Histonet] RE: GI, Uro, or Derm Path Lab Set Up
To: Sally Price <sprice2003 <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<d5e788261002050359j4521efb7x168c9a79ec399735 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
These pod labs are a curse and make it that much harder for HTs and HTLs
to get good paying, respected positions. Hospitals and reference labs are
losing their biopsy workload to GI, GU, and Derm practices.
Thomas is right. Some of these consultants leave copies of their
credentials on the wall, and the practice then trains a histo monkey to turn
out the work for $10./hr. When an inspection rolls around, oops, the HT is
"on vacation", and the histo monkey goes back to sweeping the floors or
filing, or whatever they were doing before, for the duration of the
inspection.
Pod labs are based on pure greed, and are part of the reason for
spiralling health care costs in the U.S. Any histopathology technician or
technologist that works for these consultants should realize that they are
cutting their own throat in the long run. I notice that Timothy lists a
Masters of Health Administration, but NOT an HT or HTL. Obviously he is
familiar with health care economics, but that's not the whole picture.
Money should never be the primary motivation in the practice of
medicine.
Sincerely,
Jay A. Lundgren
MS, HTL (ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 9
Date: Fri, 5 Feb 2010 12:20:01 -0500
From: Teri.Hallada <@t> midmichigan.org
Subject: [Histonet] Temperature and Humidity Monitoring System
To: histonet <@t> pathology.swmed.edu
Message-ID:
<8839B08E3ED7364E8CBBD53882C984D5141029B6 <@t> MAILSRV01.midmichigan.net>
Content-Type: text/plain; charset=us-ascii
Anyone recently price one of these for a small to medium lab?
Teresa Hallada BS, MT/CT (ASCP)
Pathology Lead
MidMichigan Health - Gratiot
teri.hallada <@t> midmichigan.org
989.463.1101 ext 3423
___________________________________
Please note that this email message and any attachments may contain
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reader of this message is not the intended recipient or the employee or
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------------------------------
Message: 10
Date: Fri, 5 Feb 2010 09:23:34 -0800
From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: RE: [Histonet] Slide baking before IHC
To: "Pat Laurie" <foreightl <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1AAF670737F193429070841C6B2ADD4C0121DF474F <@t> EXMBMCB15.ucsfmedicalcenter.org>
Content-Type: text/plain; charset=us-ascii
Here is the reference:
Effect of Slide Drying at 80Deg C on Immunohistochemistry
A.F. Henwood
J Histotechnology, VOl. 28, no. 1, March 2005
If you are an NSH member you can email the NSH office and ask for a pdf
reprint.
The author compares heating the slides at 80C for seven hours to one hour at
65C. Some antigens were adversely affected, some were not.
Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pat Laurie
Sent: Friday, February 05, 2010 12:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slide baking before IHC
Histonet,
I have heard rather anecdotally that if you bake slides at high temps (75
degrees to 80 degrees) before IHC for a long period of time (several hours
to days), you may affect antigenicity for some antibodies. Has there been
any study done about this? Also, what if it is a high temp (around 75
degrees C) for just 20 minutes? Or a low temperature for a long period of
time?
Thanks in advance for your input.
--
Patrick Laurie HT(ASCP)QIHC
plaurie <@t> cellnetix.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Fri, 5 Feb 2010 10:31:46 -0700
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] Temperature and Humidity Monitoring System
To: <Teri.Hallada <@t> midmichigan.org>, <histonet <@t> pathology.swmed.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B100BF0 <@t> server.PremierLab.local>
Content-Type: text/plain; charset="us-ascii"
VWR has thermometers that also measure humidity - these are traceable,
that's what we use to record daily temps and humidity. We have them in
each of the rooms in the lab. The catalog number is 62344-734 they cost
about $30.00 each. You can have them recalibrated yearly if you want to
but sometimes it's just cheaper to purchase new ones before the
calibration expires.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Teri.Hallada <@t> midmichigan.org
Sent: Friday, February 05, 2010 10:20 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Temperature and Humidity Monitoring System
Anyone recently price one of these for a small to medium lab?
Teresa Hallada BS, MT/CT (ASCP)
Pathology Lead
MidMichigan Health - Gratiot
teri.hallada <@t> midmichigan.org
989.463.1101 ext 3423
___________________________________
Please note that this email message and any attachments may contain
privileged and confidential information that is protected against use or
disclosure under federal and state law. The information is intended
only for the personal and confidential use of the intended recipient. If
the reader of this message is not the intended recipient or the employee
or agent responsible for delivering it to the intended recipient, you
are hereby notified that you have received this information in error and
that any review, dissemination, distribution, copying or action taken in
reliance on the contents of this communication is strictly prohibited.
If you have received this email in error, please advise by immediate
reply.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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Histonet mailing list
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