[Histonet] Slide baking before IHC

[Morken, Tim]

Here is the reference:

Effect of Slide Drying at 80Deg C on Immunohistochemistry
A.F. Henwood
J Histotechnology, VOl. 28, no. 1, March 2005

If you are an NSH member you can email the NSH office and ask for a pdf reprint.

The author compares heating the slides at 80C for seven hours to one hour at 65C. Some antigens were adversely affected, some were not.



Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pat Laurie
Sent: Friday, February 05, 2010 12:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slide baking before IHC

Histonet,

I have heard rather anecdotally that if you bake slides at high temps (75
degrees to 80 degrees) before IHC for a long period of time (several hours
to days), you may affect antigenicity for some antibodies.  Has there been
any study done about this?  Also, what if it is a high temp (around 75
degrees C) for just 20 minutes?  Or a low temperature for a long period of
time?

Thanks in advance for your input.

-- 
Patrick Laurie HT(ASCP)QIHC
plaurie <@t> cellnetix.com
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