[Histonet] Slide baking before IHC

[Pat Laurie]

Histonet,

I have heard rather anecdotally that if you bake slides at high temps (75
degrees to 80 degrees) before IHC for a long period of time (several hours
to days), you may affect antigenicity for some antibodies.  Has there been
any study done about this?  Also, what if it is a high temp (around 75
degrees C) for just 20 minutes?  Or a low temperature for a long period of
time?

Thanks in advance for your input.

-- 
Patrick Laurie HT(ASCP)QIHC
plaurie <@t> cellnetix.com