[Histonet] TRAP assay - acid phosphatase

[Liz Chlipala]

We have used it on FFPE EDTA decaled mouse bone, with a little bit of fiddling with the protocol it works pretty good
 
Liz

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Sherwood, Margaret 
Sent: Wed 2/3/2010 3:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] TRAP assay - acid phosphatase 



Has anybody  used the TRAP assay kit from Sigma for bone?  We want to use it on
mouse tibia for IHC.

If so, 1) can you do it on paraffin and/or frozen sections?
       2) if you do it on frozens, do you decalcify first?
       3) what type of blade do you use for sectioning on the cryostat? 
       3) what procedure do you follow after sectioning:  do you fix slides
(i.e. acetone, etc.)

Thank you.

Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDW 214)
Massachusetts General Hospital
55 Fruit Street
Boston, Massachusetts 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org



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