[Histonet] Comparison of AEC chromogens

[gayle callis]

Dear All, 

 

How does DAKO AEC+ compare to the new immPACT peroxidase substrate (AEC)
from Vector in terms of sensitivity, or as Dr. van der Loos would say,
efficiency?  Is your primary antibody concentration comparatively the same
with both of these AEC products?     We have enjoyed excellent results with
AEC+ over the years or with van der Loos AEC recipe- allowing low antibody
concentrations with our murine CD markers.    

 

We are not interested in DAB since we prefer a tinctorial red color of the
AEC(cells) with blue(nuclei/tissue) for contrast.    

 

We are always interested in trying new chromogen kits available on the
market.  

 

Gayle M. Callis

HTL/HT/MT(ASCP) 

Bozeman MT