[Histonet] Rapid liver core biopsy processing

[Tony Henwood]

Garth,

We have two methods available:

The first is a microwave method using the Milestone Mega TT. It uses 10%
NBF (25min at 35oC followed by 25min at 50oC), followed by an ethanol
rinse (2-3min room temp), 7min at 69oC in isopropanol and finally 8 min
at 79oC in wax.

The second uses our routine processor, 1hr in NBF at 40oC, followed by
ethanol 10min x 4, xylene 10min x3, wax 10min x3.

I have found it important to extend the NBF fixation step as much as I
can - gives better results.

Also these methods apply to core biopsies or very thin slices (<1mm)

Hope this helps

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Garth
Fraga
Sent: Friday, 27 August 2010 3:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Rapid liver core biopsy processing


Dear histonetters,
 
We do about a hundred liver transplants/year at our hospital, and the
hepatologists do lots of liver core biopsies to rule out rejection.
They want a same-day diagnosis on these, so historically they have been
rush processed on a two-hour cycle (VIP machine).  They are brought over
directly from the liver biopsy suite immediately after biopsy, so they
get very little fixation in the specimen container prior to going on the
processor.  Recently we had a couple of sub-par cases and have moved to
a four-hour processing cycle.  Do any of you have any experience dealing
with rush-processed liver cores in transplant patients?  What sort of a
processing schedule do you recommend?  Anyone handling them like frozen
sections?
 
Thanks,
 
Garth Fraga (pathologist)
University of Kansas Medical Center
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