[Histonet] Cleaning molds

Joe Nocito jnocito <@t> satx.rr.com
Fri Aug 27 08:53:00 CDT 2010

yeah, I'd be careful of this method. We used to do this at the U.S. Air 
Force hospital I used to work at when I was active duty. One time, the fire 
alarm went off and we had to evacuate the lab. Well, there must have been a 
real problem because we were out of the lab a long time. By the time we got 
the "all clear" to go back into the lab, I was the first one back and ran 
into some firemen. Seems the water boiled out of the pot and the paraffin 
was smoking up a storm.. No one said anything, but I bet that was why we 
were out of the lab so long. Now, those of you that have worked in a 
military or government hospital realize all the freaking paperwork that had 
to be filled out from that one incident. We were ordered by Safety to 
develop another method. So, we soak our molds in xylene for a couple of 
hours, covered of course as not expose the lab to the xylene fumes (that 
would required addition freaking paperwork) followed by a 100% etoh rinse, 
followed by air drying.

----- Original Message ----- 
From: <chapcl <@t> yahoo.com>
To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>; 
<histonet-bounces <@t> lists.utsouthwestern.edu>; 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, August 26, 2010 11:16 AM
Subject: Re: [Histonet] Cleaning molds

> Back when I was using metal cassette lids and an autotechnicon (so no 
> cleaning in the processor) I would throw all the lids and molds into a pot 
> after embedding. Throw a bit of laboratory detergent in, fill with water, 
> and boil on a hot plate. Once I got to a rolling boil, I would turn the 
> heat off. By the end of the day it was cool.  Peel the paraffin off the 
> top, then rinse the molds.
> Worked great!  Didn't even need mold release.
> Will
> Sent from my Verizon Wireless BlackBerry
> -----Original Message-----
> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
> Sender: histonet-bounces <@t> lists.utsouthwestern.edu
> Date: Thu, 26 Aug 2010 10:53:58
> To: 'histonet <@t> lists.utsouthwestern.edu'<histonet <@t> lists.utsouthwestern.edu>
> Subject: [Histonet] Cleaning molds
> Does anyone in histoland clean their embedding molds in a dishwasher? 
> Otherwise, besides placing in the cleaning cycle of your processor, how do 
> sites clean their molds??  Simple, but plaguing question!!!!!!!  Thanks 
> all!
> Dorothy Webb
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