[Histonet] cytokeratin 18 in liver
PALMER Jason (SVHM)
Jason.PALMER <@t> svhm.org.au
Thu Aug 26 22:31:39 CDT 2010
Just wondering if anyone has experience with cytokeratin 18 immunohistochemistry labelling in liver (FFPE)? I have used an Abcam rabbit anti human CK18 to try and label hepatocytes in both human and mouse adult liver and have found that I get strong labelling in bile duct epithelia but essentially nothing in hepatocytes. I say essentially nothing, but there does appear to be faint possible specific staining in a few hepatocytes. From my reading, I had thought that cytokeratin 18 (and 8) are abundantly expressed in hepatocytes and should be easy to label, but that is not what I have found.
We are trying to tissue engineer liver from progenitor cells in a mouse model, and need to be able to identify these cells, whether liver progenitors or mature / differentiated hepatocytes, hence my choice of an antibody against CK18 which should label both. We have also used a pan cytokeratin (Dako Z0622) with mixed results and were hoping for something a little more defined.
Any thoughts greatly appreciated,
Histology Laboratory Coordinator
42 Fitzroy St, Fitzroy Victoria 3065
tel +61 3 9288 4045
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email: jason.palmer <@t> svhm.org.au
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