[Histonet] Re: [IHCRG] PAX-5 - B-cell specific activator protein (BSAP)

Richard Cartun Rcartun <@t> harthosp.org
Wed Aug 25 18:15:37 CDT 2010

We use a mouse mAb (clone 24) from BD Biosciences on Leica-Microsystems' Bond with absolutely no problems.


Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

>>> "Taylor, Jean" <jtaylor <@t> meriter.com> 8/19/2010 10:44 AM >>>
Hi Everyone,

I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP).


Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI

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Subject: Histonet Digest, Vol 81, Issue 23

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Today's Topics:

   1. RE: CD34 positive control (McMahon, Loralee A)
   2. Ventana Renaissance Containers (ricky hachy)
   3. CD34 Control (Silverman, Jeffrey)


Message: 1
Date: Wed, 18 Aug 2010 12:17:39 -0400
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: RE: [Histonet] CD34 positive control
To: Joel Reichensperger <jreichensperger <@t> siumed.edu>, Histonet
        <histonet <@t> lists.utsouthwestern.edu>
        <C27AA2A01CEF31469813089E226F582E02D5A7CAA4 <@t> URMCMS7.urmc-sh.rochester.edu>

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Just about any tissue should work.   It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
We use a sausage style control here with about 30 different types of tissue.

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensperger <@t> siumed.edu] 
Sent: Wednesday, August 18, 2010 12:05 PM
To: Histonet
Subject: [Histonet] CD34 positive control

  I have a doctor who wants to stain some tissue with cd34. I need to
know if anyone can recommend a good positive control tissue for this
antibody. The staining will be done in paraffin embedded sections.
Thanks in advance.


Joel Reichensperger
Researcher II
Southern Illinois University
Plastic Surgery Institute
jreichensperger <@t> siumed.edu 
217-545-7309 (Office)
217-545-1824 (Fax)

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Message: 2
Date: Wed, 18 Aug 2010 16:28:39 +0000
From: ricky hachy <elciba <@t> hotmail.com>
Subject: [Histonet] Ventana Renaissance Containers
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT114-W54AB5F08E7E0CFC97193EDCF9D0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Hello ,

I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE .

Anyone could sell me ?




Message: 3
Date: Wed, 18 Aug 2010 12:30:58 -0400
From: "Silverman, Jeffrey" <JSilverman <@t> NSHS.edu>
Subject: [Histonet] CD34 Control
To: "'jreichensperger <@t> siumed.edu'" <jreichensperger <@t> siumed.edu>
Cc: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
        <83F4D81747A7094DAFE3AE87151ECB941CE41442A3 <@t> SYKECHXVS01.nslijhs.net>
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A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis,  are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels.

Jeff Silverman


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