[Histonet] Re: Histonet Digest, Vol 79, Issue 38
Chris Evanish
cevanish <@t> mont-hosp.com
Wed Aug 25 13:13:50 CDT 2010
Help!!
I use a Ventana Benchmark XT. Recently I have gotten a brown granular
staining of cytoplasm, not as dark as true specific staining.
- Apocrine metaplasic cells.
- Non-specifically staining neoplastic prostatic
epithelial cytoplasm.
Benign prostatic cytoplasm on prostate bx using CK 34BETA12, but not
just that antibody as it also appeared in breast tissue on ER.
At first I considered it was Endogenous Biotin, so I ran multiple
prostate biopsy slides with a blocker but they did not improve. However
a strange thing occurred on that run. On two of the slides, one section
of tissue stained good, but another section of tissue on the same slide
did not stain well.
I hope maybe somebody has an idea or two that will help.
Thanks,
Chris
Chris D. Evanish
Histology Supervisor
Montgomery Hospital
610-270-2379
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Today's Topics:
1. Canine bone sectioning trouble (Schneider,Lynda S)
2. RE: Coverslips (Malika Benatti)
----------------------------------------------------------------------
Message: 1
Date: Tue, 29 Jun 2010 12:17:58 -0400
From: "Schneider,Lynda S" <emlynda <@t> pathology.ufl.edu>
Subject: [Histonet] Canine bone sectioning trouble
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<94A0BFCE84CB0F4A84C50EAC9B02D0A9019423208F <@t> HSC-CMS01.ad.ufl.edu>
Content-Type: text/plain; charset="us-ascii"
Hello out there...
We are having trouble sectioning canine bone. The samples are bone
marrow cubes approximately 2cm thick and 1in wide. They were fixed for
15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid
decalcifier. They were faced in and then surface decaled again for
about 30 mins. When sectioned, much of the marrow was missing and the
bone was torn and shredded. We thought that maybe the samples had not
been fixed sufficiently so refixed overnight in 10% NBF again. The
samples were then reprocessed as they had been originally. The
processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH
x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each,
paraffin x 4, 40 mins each. Again they were faced in and decaled for
another 30 mins or so. This time as soon as the sections are placed on
the water bath (38 degrees) they explode and/or come apart so severely
sections can almost not be picked up. If sections are even obtainable
they are of horrible quality. Does anyone have any suggestions? Thank
you so much in advance!
Lynda
------------------------------
Message: 2
Date: Tue, 29 Jun 2010 17:53:26 +0100
From: "Malika Benatti" <BenatM <@t> gosh.nhs.uk>
Subject: [Histonet] RE: Coverslips
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4C2A3319.4626.0038.0 <@t> gosh.nhs.uk>
Content-Type: text/plain; charset=UTF-8
** Proprietary **
** Reply Requested When Convenient **
Hi there,
We use the CellPath CoverSlips 22x50mm No 1.5 they are great for hand
mounting but when used with the Leica CV5030 they are a real pain.
most of the CoversSlips get rejected.
If anyone use the same equipment, let me know how you are getting one
.
Cheers,
Malika
------------------------------
Message: 7
Date: Mon, 28 Jun 2010 15:06:08 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] RE: Coverslips
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92AD9B20A6C38C4587A9FEBE3A30E164015DFD5C93 <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
I forgot to say that the packaging has changed to their Histo Hippo
theme, but they assure me they are the same product. j
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Weems, Joyce
Sent: Monday, June 28, 2010 14:29
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Coverslips
For years we have used coverslips from Mercedes - the ones in a plain
white box labeled in German. This year we have begun to have problems
with them being dirty and having bits of glass on them that cause air
bubbles when used on the automatic coverslipper. Is anyone else having
this problem? They have worked with us to try to resolve it, and have
replaced several boxes, but the replacements are doing the same thing.
Thanks for your input, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
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------------------------------
Message: 8
Date: Mon, 28 Jun 2010 12:21:33 -0700
From: "Kathleen Boozer" <BoozerKA <@t> ah.org>
Subject: Re: [Histonet] Coverslips
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Joyce Weems"
<JWeems <@t> sjha.org>
Message-ID: <4C2893CC.4AA8.00C0.1 <@t> ah.org>
Content-Type: text/plain; charset=US-ASCII
I am having the same issues and we purchased the expensive "Platinum"
to avoid that problem. They just sent me replacements and I haven't
tested them all yet.
Kathy Boozer, HT (ASCP), IHCQ
Adventist Medical Center
10123 SE Market St.
Portland, OR 97216
boozerka <@t> ah.org
Malika Benatti
Specialist BMS
Camelia Botnar Laboratories
Histopathology Department
Great Ormond Street Hospital
London WC1N 3JH
Tel: +44 20 7405 9200 ext 5475
Fax: +44 20 7829 7875
benatm <@t> gosh.nhs.uk
>>> "Weems, Joyce" <JWeems <@t> sjha.org> 6/28/2010 11:28 AM >>>
For years we have used coverslips from Mercedes - the ones in a plain
white box labeled in German. This year we have begun to have problems
with them being dirty and having bits of glass on them that cause air
bubbles when used on the automatic coverslipper. Is anyone else having
this problem? They have worked with us to try to resolve it, and have
replaced several boxes, but the replacements are doing the same thing.
Thanks for your input, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
Confidentiality Notice:
This e-mail, including any attachments is the
property of Catholic Health East and is intended
for the sole use of the intended recipient(s).
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confidential. Any unauthorized review, use,
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not the intended recipient, please delete this message, and
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------------------------------
Message: 9
Date: Mon, 28 Jun 2010 15:36:22 -0400
From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
Subject: RE: [Histonet] RE: Coverslips
To: "Weems, Joyce" <JWeems <@t> sjha.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<073AE2BEA1C2BA4A8837AB6C4B943D9703E2441E <@t> PHSXMB30.partners.org>
Content-Type: text/plain; charset="us-ascii"
We have been using them (24x50) and have had no problems with broken
glass
pieces. I do agree that they seem "dirty".
Never contacted them about it.
Peggy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Monday, June 28, 2010 3:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Coverslips
I forgot to say that the packaging has changed to their Histo Hippo
theme, but
they assure me they are the same product. j
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Monday, June 28, 2010 14:29
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Coverslips
For years we have used coverslips from Mercedes - the ones in a plain
white box
labeled in German. This year we have begun to have problems with them
being
dirty and having bits of glass on them that cause air bubbles when
used
on the
automatic coverslipper. Is anyone else having this problem? They have
worked
with us to try to resolve it, and have replaced several boxes, but the
replacements are doing the same thing. Thanks for your input, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
Confidentiality Notice:
This e-mail, including any attachments is the property of Catholic
Health East
and is intended for the sole use of the intended recipient(s).
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If
you are
not the intended recipient, please delete this message, and reply to
the sender
regarding the error in a separate email.
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Message: 10
Date: Mon, 28 Jun 2010 12:41:44 -0700
From: sgoebel <@t> xbiotech.com
Subject: RE: [Histonet] RE: Coverslips
To: "Weems,Joyce" <JWeems <@t> sjha.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
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Content-Type: text/plain; charset="utf-8"
I just got a box of these as a "trial". They all have been far
(used 1/2 the box so far)? Maybe there is gunk in your co
verslipper that is causing this or possibly some glass particles? I
c
Sarah Goebel, B.A., HT (ASCP)
<
Histotechnicia
XBiotech USA Inc.
<
Austin, Texas 78744
<
(512)
-------- Original Message --------
Subject: [Histonet] RE: Coverslips
From: "Weems, Joyce" <[1]JWeems <@t> sjha.o Date: Mon, June 28, 2010
12:06 pm
To: "[2]histonet <@t> lists. <[3]histonet <@t> lists.u I forgot to say
that the packaging has changed to their Histo Hippo
theme, -----Original Message-----
From: [4]histon
[[5]mailto:histonet-bounces <@t> lists.utsouthwestern.edu Weems, Joyce
Sent: Monday, June 28, 2010 14:29
To: [6]histonet <@t> lists.u Subject: [Histonet] Coverslips
For years we have used coverslips from Mercedes - the ones in a
plain
white with them air bubbles when us having this problem?
They and have replaced several boxes, same thing. Thanks for
your
input, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
Confidentiality Notice:
This e-mail, including any attachments is the property of
Catholic
Health E recipient(s).
It may contain information that is privileged and confidential.
Any
unauth If you are n and reply to the sen
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[7]Histonet <@t> lists.utsou [8]http: Confidentiality Notice:
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[9]Histonet <@t> lists.utsou [10]http:
References
1. 3D"mailto://JWeems@sjha.org"/
2. 3D"mailto://histonet@lists.utsouthwestern.edu"/
3. 3D"mailto://histonet@lists.utsouthwestern.edu"/
4. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/
5. 3D"mailto:histonet-bounces 6.
3D"mailto://histonet@lists.utsouthwestern.edu"/
7. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
8. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
9. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
------------------------------
Message: 11
Date: Mon, 28 Jun 2010 14:48:44 -0500
From: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>
Subject: RE: [Histonet] Tissue Processors
To: "'Feher, Stephen'" <sfeher <@t> CMC-NH.ORG>, mohamed abd el razik
<k84as <@t> yahoo.com>, "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A2CC <@t> EXCHMBC2.ad.ah.local>
Content-Type: text/plain; charset="iso-8859-1"
I second Steve's comments about the Peloris.
Jan Mahoney
Omaha
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Feher, Stephen
Sent: Monday, June 28, 2010 1:56 PM
To: mohamed abd el razik; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Tissue Processors
We have 2 Peloris processors and run protocols ranging from 2 hrs to
overnight. Several 2 and 4 hour protocols daily keeps a constant flow
of specimens moving through the lab for those interested in LEAN small
batch processing. As far as programmability, it 's all touch screen
driven and easy. It's a smart processor so we don't change all
solutions weekly. We only change the ones that are needed. The
parameters that determine how long to use a particular solution are
also
programmable. Changing solutions is done by pumping out of the
individual bottles and into waste containers and refilling is pumped
from clean containers of solution back into the original containers.
No
heavy lifting required.
Ours are in a separate room from our microtomy area so we hooked a
simple door bell up to the local alarm jack on the back. When
processing is done, we get the appropriate tone. Remote alarm is also
tied in to the hospital switchboard in case a processor goes down at
night or during the weekend.
We really like the versatility and dependability of these processors.
Steve
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
mohamed abd el razik
Sent: Saturday, June 26, 2010 3:41 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: Fw: [Histonet] Tissue Processors
i need it too as we are going to bring new tissue processor to our
small lab
--- On Sat, 6/26/10, Shirley Pan <sj_pan <@t> yahoo.com> wrote:
From: Shirley Pan <sj_pan <@t> yahoo.com>
Subject: [Histonet] Tissue Processors
To: histonet <@t> lists.utsouthwestern.edu
Date: Saturday, June 26, 2010, 7:55 AM
We are in the process of trying out tissue processors. Are there any
users of the Leica Peloris or Thermo EG who can help us out with some
opinions? Reliability, ease of changing solutions, programmability?
Thanks for any help.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 12
Date: Mon, 28 Jun 2010 16:04:27 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] RE: Coverslips
To: "sgoebel <@t> xbiotech.com" <sgoebel <@t> xbiotech.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92AD9B20A6C38C4587A9FEBE3A30E164015DFD5CBA <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
We can coverslip by hand, but soon after the slides are coverslipped
on
the machine, air bubbles begin to form. We we coverslip them by hand,
there is visible glass that we can feel and move if we need too.
They've
been good for so long, I want to make sure it's not just us.
Thanks for the feedback! j
________________________________
From: sgoebel <@t> xbiotech.com [mailto:sgoebel <@t> xbiotech.com]
Sent: Monday, June 28, 2010 15:42
To: Weems, Joyce
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Coverslips
I just got a box of these as a "trial". They all have been fine so
far
(used 1/2 the box so far)? Maybe there is gunk in your coverslipper
that is causing this or possibly some glass particles? I coverslip by
hand.
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
-------- Original Message --------
Subject: [Histonet] RE: Coverslips
From: "Weems, Joyce" <JWeems <@t> sjha.org<mailto://JWeems@sjha.org>>
Date: Mon, June 28, 2010 12:06 pm
To:
"histonet <@t> lists.utsouthwestern.edu<mailto://histonet@lists.utsouthwestern.edu>"
<histonet <@t> lists.utsouthwestern.edu<mailto://histonet@lists.utsouthwestern.edu>>
I forgot to say that the packaging has changed to their Histo Hippo
theme, but they assure me they are the same product. j
-----Original Message-----
From:
histonet-bounces <@t> lists.utsouthwestern.edu<mailto://histonet-bounces@lists.utsouthwestern.edu>
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Monday, June 28, 2010 14:29
To:
histonet <@t> lists.utsouthwestern.edu<mailto://histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Coverslips
For years we have used coverslips from Mercedes - the ones in a plain
white box labeled in German. This year we have begun to have problems
with them being dirty and having bits of glass on them that cause air
bubbles when used on the automatic coverslipper. Is anyone else having
this problem? They have worked with us to try to resolve it, and have
replaced several boxes, but the replacements are doing the same thing.
Thanks for your input, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
Confidentiality Notice:
This e-mail, including any attachments is the property of Catholic
Health East and is intended for the sole use of the intended
recipient(s).
It may contain information that is privileged and confidential. Any
unauthorized review, use, disclosure, or distribution is prohibited.
If
you are not the intended recipient, please delete this message, and
reply to the sender regarding the error in a separate email.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu<mailto://Histonet@lists.utsouthwestern.edu>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
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for the sole use of the intended recipient(s).
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not the intended recipient, please delete this message, and
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
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not the intended recipient, please delete this message, and
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------------------------------
Message: 13
Date: Mon, 28 Jun 2010 13:19:18 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Unstained Slides
To: histonet <@t> lists.utsouthwestern.edu,
Rick.Garnhart <@t> memorialhealthsystem.com
Message-ID: <946724.39272.qm <@t> web65713.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Depending on theáepitope you are trying to detect, the time could be
limitedáfrom less than one month to 1-2 years.
RenÎ̃ J.
--- On Mon, 6/28/10, Rick.Garnhart <@t> memorialhealthsystem.com
<Rick.Garnhart <@t> memorialhealthsystem.com> wrote:
From: Rick.Garnhart <@t> memorialhealthsystem.com
<Rick.Garnhart <@t> memorialhealthsystem.com>
Subject: [Histonet] Unstained Slides
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, June 28, 2010, 11:54 AM
Histoland, Howá is everyone storing/filing unstained slide. And how
long are they good for to use for immunohistochemistry.
Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph:á 719-365-6926
Fax: 719-365-6373
rick.garnhart <@t> memorialhealthsystem.com
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Vision: To create an outstanding health system where patients heal and
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------------------------------
Message: 14
Date: Mon, 28 Jun 2010 14:58:48 -0600
From: Jay Lundgren <jaylundgren <@t> gmail.com>
Subject: [Histonet] Inexpensive fume hood?
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AANLkTilUMvunMyVABAV6JngjGWa9KUWfnZWfW7rnnn1v <@t> mail.gmail.com>
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Can anyone recommend an inexpensive fume hood/ workstation? Not the
full on
metal type that hangs from the ceiling, but the Lucite bench top unit
with a
carbon filter. I want to avoid the capital equipment rigmarole, so it
has
to cost less than $1000. It is to put a small automated coverslipper
under, 16" H x 20" W x 10" D .
Thanks,
Jay A. Lundgren, M.S., HTL (ASCP)
------------------------------
Message: 15
Date: Mon, 28 Jun 2010 14:04:42 -0700
From: Pat Laurie <foreightl <@t> gmail.com>
Subject: Re: [Histonet] Tissue Processors
To: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AANLkTimxFg5ZsMUCwpM8NRVZWYX2__LWESDDgZ_H-yh4 <@t> mail.gmail.com>
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I 3rd the previous statements about the Peloris. I had 2 pelori at my
previous job, and now we currently have 3 pelori. We are also looking
at
getting a 4th. We use them regularly and due to the ability to use
shorter
programs, we run about 15 runs a day between them while still having
significant downtime. I must warn you though, it is necessary to keep
the
service contracts on them. They are about 99% computer and have
a significant number of moving parts, but they do work wonderfully.
On Mon, Jun 28, 2010 at 12:48 PM, Mahoney,Janice A <
Janice.Mahoney <@t> alegent.org> wrote:
> I second Steve's comments about the Peloris.
> Jan Mahoney
> Omaha
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Feher,
Stephen
> Sent: Monday, June 28, 2010 1:56 PM
> To: mohamed abd el razik; Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Tissue Processors
>
> We have 2 Peloris processors and run protocols ranging from 2 hrs to
> overnight. Several 2 and 4 hour protocols daily keeps a constant
flow of
> specimens moving through the lab for those interested in LEAN small
batch
> processing. As far as programmability, it 's all touch screen
driven
and
> easy. It's a smart processor so we don't change all solutions
weekly. We
> only change the ones that are needed. The parameters that determine
how
> long to use a particular solution are also programmable. Changing
solutions
> is done by pumping out of the individual bottles and into waste
containers
> and refilling is pumped from clean containers of solution back into
the
> original containers. No heavy lifting required.
>
> Ours are in a separate room from our microtomy area so we hooked a
simple
> door bell up to the local alarm jack on the back. When processing
is
done,
> we get the appropriate tone. Remote alarm is also tied in to the
hospital
> switchboard in case a processor goes down at night or during the
weekend.
>
> We really like the versatility and dependability of these
processors.
>
>
> Steve
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of mohamed abd
el
> razik
> Sent: Saturday, June 26, 2010 3:41 PM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: Fw: [Histonet] Tissue Processors
>
>
> i need it too as we are going to bring new tissue processor to our
small
> lab
> --- On Sat, 6/26/10, Shirley Pan <sj_pan <@t> yahoo.com> wrote:
>
>
> From: Shirley Pan <sj_pan <@t> yahoo.com>
> Subject: [Histonet] Tissue Processors
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Saturday, June 26, 2010, 7:55 AM
>
>
> We are in the process of trying out tissue processors. Are there any
users
> of the Leica Peloris or Thermo EG who can help us out with some
opinions?
> Reliability, ease of changing solutions, programmability? Thanks for
any
> help.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> _______________________________________________
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> _______________________________________________
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>
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--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie <@t> cellnetix.com
------------------------------
Message: 16
Date: Mon, 28 Jun 2010 17:57:37 -0500
From: "Amador, Amanda" <aamador <@t> ameripath.com>
Subject: [Histonet] Modified Genta
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1D9B439FC446244095353DCA4FC23DD20454E1D27B <@t> MWNMAIL00.ameripath.local>
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Is there someone that could help out with the Modified Genta? We
tried
it in the microwave and we are not sure if we are doing it correctly.
We would appreciate any sample procedures to see if we need to change
something.
Thanks to anyone who can help out.
aamador <@t> ameripath.com<mailto:aamador <@t> ameripath.com>
------------------------------
Message: 17
Date: Tue, 29 Jun 2010 09:53:10 -0400
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] what do you think?
To: mohamed abd el razik <k84as <@t> yahoo.com>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <4C29FAC6.1030909 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Greetings Mohamed:
The lamina propria brings small blood vessels and lymphatics close to
the epithelium lining the large and small intestines. This allows
nutrients to be passed back and forth and for the immune system to
monitor any antigens that cross the epithelium. The relationship
between
the immune system and the contents of the GI tract is very complex.
Lymph nodules in the lamina propria would make it thicker in some
regions than in other regions. Whether the thickening you refer to is
pathological or just the normal response to a variety of factors is
difficult to say.
Geoff
mohamed abd el razik wrote:
> dear histonetters
> i have a quistion please regarding the thickness of lamina propria
in
small or larg intestine.
> what is the significant of it?? does it mean inflamation and
pathological condition or mean more size and so absorption of
nutrients?
i mean does the thickning is favorable or not?
>
> thanks
> Mohamed Abd Elrazik
> Histology dep.
> Fac. of Vet. Med.
> Cairo . Univ. -Egypt
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 18
Date: 29 Jun 2010 13:59:09 -0000
From: "abijag " <abijag76 <@t> rediffmail.com>
Subject: [Histonet] Your experience with microm HMS 740 auto stainer
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1277819686.S.1418.34634.webmail.rediff.com.drafts.1277819949.50235 <@t> webmail.rediffmail.com>
Content-Type: text/plain; charset="UTF-8"
Dear Histonetters,
We would like to procure one tissue auto stainer for our histology
lab(mainly H&E staining) In this respect, I request your experience
about Microm HMS 740 automatic stainer. Please share your comments
regarding the staining reproducibility,ease of handling and their
claim
about drip prevention technology. Based on your valuable feedback, we
will make our decision.
Thanks for all help
Abi jagannath
------------------------------
Message: 19
Date: Tue, 29 Jun 2010 08:20:32 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Inexpensive fume hood?
To: histonet <histonet <@t> lists.utsouthwestern.edu>, Jay Lundgren
<jaylundgren <@t> gmail.com>
Message-ID: <364541.78070.qm <@t> web65712.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Check a Fisher catalog. The ones they sell are good for your purposes.
RenÎ̃ J.
--- On Mon, 6/28/10, Jay Lundgren <jaylundgren <@t> gmail.com> wrote:
From: Jay Lundgren <jaylundgren <@t> gmail.com>
Subject: [Histonet] Inexpensive fume hood?
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, June 28, 2010, 4:58 PM
Can anyone recommend an inexpensive fume hood/ workstation?á Not the
full on
metal type that hangs from the ceiling, but the Lucite bench top unit
with a
carbon filter.á I want to avoid the capital equipment rigmarole, so
it
has
to cost less than $1000.á It is to put a small automated coverslipper
under,á 16" H x 20" W x 10" D .
á á á á á á á á á á á á á á á á á á á á á á á á
á á á á á á á á
áááThanks,
Jay A. Lundgren, M.S., HTL (ASCP)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 20
Date: Tue, 29 Jun 2010 08:53:32 -0700
From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: RE: [Histonet] New CAP question ANP.22760
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1AAF670737F193429070841C6B2ADD4C021C3610F1 <@t> EXMBMCB15.ucsfmedicalcenter.org>
Content-Type: text/plain; charset=us-ascii
Thomas and Daniel both make good points.
While clinical labs do run measurable (quantitative) tests they are
also running completely closed systems in which all reagents are from
a
certain vendor. All the reagents are validated by a vendor and all
reagents are validated and calibrated for that system.
Running qualitative tests is only different at the interpretive stage.
All other stages should be standardized and validated to ensure the
system works as intended every day, every test.
IHC, of course, is a whole different ballgame. There probably is not a
single lab out there that uses only one vendor and uses only one
system
to do IHC testing. Even if you use one automated staining system you
may
use antibodies from a different vendor, or you may do something
outside
the system that is very different than other labs use. There is such
disparity between testing in different labs that it is difficult to
compare results and even techniques between labs. That is the crux of
the issue and what CAP and others are trying to address by tightening
the validation screws. In the near future labs will need to have very
robust validation and documentation for all antibodies in order to
prove
they are doing things correctly. That scenario is already here for the
breast markers. It will come for the others as well.
Right now the instances in which solid validation methods are
absolutely necessary is when validating the breast markers ER, PR and
HER2, which all are used as stand-alone tests to determine treatment,
and for ASR's. If you use a vendor kit with all reagents supplied you
must still prove it works in your lab as intended ("verify outside
data." The "outside data" is the claim that it stains in a certain
way).
You must run cases of various expressions and show your lab gets the
proper results. Third party verification is very helpful, that is,
comparing your results to that of other labs on the same material (CAP
surveys, or set up a partnership with another lab).
If you use only the antibody for one of those markers, or mix and
match
components outside a FDA-approved kit, then you are responsible for
performing a comprehensive validation of the test.
Its worth noting that every news article I've seen about
pathology/histology labs in the last several years has been about
failures to do the breast panel tests correctly. We are under a
microscope these days and it will pay off to make sure you are doing
the
tests well!
Most other markers in IHC are ancillary tests that are run in
conjunction with other tests to determine a diagnosis. Those
antibodies
still need to be validated in your system but don't require a large
sample of cases. They have been validated as IVD's by the vendor so
just
need to be shown they work as intended with your lab's methods and
instruments. But I still think it is worthwhile to do a validation
that
includes a range of expressions along with irrelevant tissue
(negative).
That will help calibrate your expectations about how the antibody
works
and give you a baseline to refer to if problems arise. Running one or
two slides to make sure it "works" is not really satisfactory.
Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Thomas Jasper
Sent: Saturday, June 26, 2010 1:45 PM
To: Daniel
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] New CAP question ANP.22760
Daniel,
I think your observations are very good. Which brings me again to a
point I was trying to make earlier. Anatomic Pathology is not the
same
as the General Clinical Lab or it's close counterparts. It almost
seems
like this is overblown re: validation. As you've pointed out, the
interpretation is up to the pathologist. Patients (prostate, i.e.)
will
correlate with other tests. When known positives demonstrate properly
you're valid. Even automated systems can use algorithms to score
strength of positivity...which varies yet will show as positive
(valid).
You receive a new batch of Ab you run it on a known positive, that's
the
bottom line.
It seems somehow CAP or clinical lab (trained) folks want more than
that. And again (as you pointed out) the appreciation for the
differences in how we operate as laboratorians seems to fall by the
wayside. As you mention pushing 20, 50, 100 is not feasible (and
frankly unnecessary). I don't know where the answer lies in making
everyone happy here. I do know that some thinking outside the box and
trying understand AP is required. We all want good patient care, but
one size does not fit all.
Thanks,
tj
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Daniel
Sent: Saturday, June 26, 2010 11:08 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] New CAP question ANP.22760
One question I think a lot of you are not considering is that the
clinical laboratory usually tests for analytes present in most
patients.
This allows the clinical lab to more easily run validations with
hundreds of patients, using statistical tools to analyze the
precision,
specificity and sensitivity of the test. What most of those concerned
with this new CAP standard (and I count among them) is that our
testing
often targets a very rare population of patients. As such, an
extensive
validation is much more difficult to establish. It then makes the
statistical models used in clinical tests much less valuble since the
accuracy of these formulas decrease with smaller sample sizes.
Additionally, the pathology of the clinical tests are reported as a
measurement which does not have an intrinsic value. It must be
interpreted by a clinician to give value to the patient. For example,
the Blood Urea Nitrogen test value range usually is between 7-20 mg/dL
while values above 50 are considered a marker of poor kidney function.
Elevated values, however, do not mean that kidney functions are
impaired
but instead may be due to other pathologies such as congestive heart
failure, gi bleeding, certain steroids and even diet.
For histopathology even relatively common antibodies such as pan-T
cell
marker CD3 usually only stain 75% of T cell neoplasms. A negative
result for that minority does not indicate that the tumor does not
have
a T cell lineage. It relies instead on the pathologist interpreting
the
result much as the clinician looks at the BUN value in relation to
other
factors in the patient's presentation. When we validate this kind of
antibody, do we perform testing then on T cells and B cells (give me a
good tonsil), on malignant samples or some combination of the two?
What
is a reasonable number of tests to run then?
If I choose 10 normal lymphatic tissue blocks for my routine T/B cell
marking (it can't be as extensive as ER/PR can it?) how many samples
should I choose for my malignant population? If I use another 10
anyone
with a background in statistics will tell you that the sample size is
too small to interpret accuracy. If I push it up to 20, 50 or 100 I
would be certain that many laboratories would not be able to afford
the
cost of the validation. This would then push many good laboratories
out
of the business of IHC with the unintended result of delaying
diagnoses
and increasing patient costs by driving testing to fewer and fewer
testing outlets.
CAP's new path with ER/PR seems to be trying to achieve a noble end of
improving quality in the laboratory but without an understanding of
the
complexities and consequences of the method they are implementing.
Dan
-----Original Message-----
>From: Jesus Ellin <JEllin <@t> yumaregional.org>
>Sent: Jun 26, 2010 6:28 AM
>To: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>,
>histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] New CAP question ANP.22760
>
>I have been reading the post to this question and it seems to me that
there are different standards depending on the lab that is operating
the
methodology. I do agree that the core lab for years have had the
instruction and training in the performance of validation. One thing
that comes to mind as well is why has histology not had this training?
Why are we not getting this from our certification agency, our
professional societies and biggest reason where is our
standardization.
It seems to me that with all these regualtions in plac for so long,
why
were we missed. Is it because when inspected through CAP we are being
inspected by a pathologist rather than a histo tech? These are some
of
the questions at hand. I to see new standards within the CAP
checklist
as well as other regulatory organizations that will affect the future
of
the Anatomic Pathology community. But I think we need is to provide a
underlying architecture for our peers, so that we can begin the
transition to the future. This is only the beginning, there is still
Digital Image Analysis and Telepathology. It funny we are looking to
become a hybrid of radiology and the core lab, but with the best of
both
worlds. Tim great structure for the validation study.
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Morken,
>Tim
>Sent: Wed 6/23/2010 9:48 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] New CAP question ANP.22760
>
>Joe,
>
>You wrote : The folks in the 'clinical' lab have been performing more
comprehensive and complex validation procedures for a very long time
..."
>
>Those were my thoughts exactly. While the person replying may or may
not have specific histology experience she will have clinical lab
experience (however, my guess is that she is exposed to histology
regularly at CAP). Clinical labs have a bit of an easier time,
actually,
because they validate primarily to known concentration controls -
analytical controls manufactured at a range of known concentrations
for
instance. The institution then adds in their normal controls for
validation.
>
>As far as the current question about validating a new lot of reagent
the best practice is to run parallel tests on the same machine. If
that
is not easily possible on a particular manufacturer's instrument then
the question should be asked of them: Why not? If this is a
requirement
the manufacturer should provide an easy path to meeting the
requirement.
However, if that is not the case then the institution simply writes a
procedure to get around the inadequacies of the instrument (Maybe the
vendor can help with that). Then follow the procedure. That should
satisfy inspectors.
>
>An "...appropriate panel of tissues..." is whatever the institution
deems appropriate for the given antibody or reagent. This is a perfect
place for tissue arrays. You can make your own or buy them.
>
>IHC must meet CLIA validation guidelines but since IHC is generally
qualitative the requirements must be understood and methods adapted to
a
qualitative scenario. Several IHC and Histotechnology books discuss
the
subject at length (Taylor, Dabbs, Bancroft for instance).
>
>Below is a brief overview of how to do that. (for more in-depth info
this was covered in an NSH teleconference I gave last year -
PowerPoint,
audio and references available from NSH-, and will be covered in a
similar workshop at NSH in Seattle this year).
>
>
>1)
>CAP General Validation
>CAP GEN.42020-42163 Test Method Validation Follows CLIA CFR Sec
>493.1253 Does not apply well to IHC (IHC is usually qualitative)
>
>But the general principle applies:
>The laboratory must have data on each test's accuracy, precision,
analytic sensitivity, interferences and reportable range.
>
>Unmodified FDA-cleared or approved tests: the lab may use
manufacturer
information or published reports but lab must verify outside data.
>
>Non-FDA cleared: Lab MUST verify or establish analytic accuracy,
precision, sensitivity, specificity and reportable range.
>
>2) Validation includes:
>Accuracy:
> Compare results with New antibody to a previously validated
antibody on the same tissues
>
>Precision:
> Test samples with varying antigen expression
> Intra-run, Inter-run tests, 10 slides each (reproducibility)
>
>Sensitivity:
> True Positive vs False Negative (higher % FN = less
sensitive)
>
>Interferences [Specificity]:
> True Negative vs False Positive (Higher % FP = less specific)
> Delineate what could interfere to give a false positive or
false negative result.
>
>Reportable Range
> Establish a scoring system
> Provide the definition of a positive result
>
>3)Sensitivity
>
>Analytic Sensitivity:
> Lowest amount of substance detectable by the test
> Can only be done with controls of known concentration
>
>Diagnostic Sensitivity:
> Ability of the test to determine true diagnostic positive
verses false negative (higher % FN = less sensitive)
> Requires comparison to a previously validated antibody
>
>IHC Sensitivity:
> Extent to which an antibody can be diluted and still achieve
target recognition. NOTE: This is determined by antibody AND
detection system!
>
>
>
>4) Specificity:
>
>Analytic Specificity
> Accuracy on tests of known positive and negative controls
> Controls of known concentration
> Determine what could "Interfere" to confound the result
>
>
>Diagnostic Specificity
> Ability of a test to determine true diagnostic negative
verses
false positives (Higher % FP = less specific)
> Requires comparison to a previously validated antibody
>
>
>IHC Specificity
> Ability of an antibody to bind exclusively to its particular
antigen in the absence of staining of other molecules
> Or, staining of other structures in addition to target
>structures/cells
>
>(Sensitivity and Specificity adapted from: Theoretical and Practical
>Aspects of Test Performance, in Immunomicroscopy, Taylor & Cote,
2005)
>
>Tim Morken
>Supervisor, Histology / IPOX
>UCSF Medical Center
>San Francisco, CA
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>JMyers1 <@t> aol.com
>Sent: Tuesday, June 22, 2010 6:51 PM
>To: tjasper <@t> copc.net
>Cc: histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] New CAP question ANP.22760
>
>Tom:
>
>As much as I agree with your acknowledgment that its seems a bit odd
>for the CAP to have a blood-banker responding to AP-related issue,
I'm
>actually not surprised. The folks in the 'clinical' lab have been
>performing more comprehensive and complex validation procedures for a
>very long time, and they wonder why IHC isn't expected to follow the
>same requirements as chemistry, immunology, etc. -- IHC is, after
all,
>an awful lot like ELISA. And rightfully so, because IHC is, under
CLIA
>(which supersedes CAP), considered highly-complex, non-waived testing
>-- and is, therefore, subject to the same Quality Systems regulations
>(in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing
performed in other areas of the lab.
>
>Could it be that, because AP produces qualitative results that are
>interpreted by a pathologist and CP produces quantitative results
that
>are interpreted by an analyzer, we somehow think that CLIA rules
don't
>apply to IHC? I certainly don't have the answer to that, but it make
>me wonder what the future holds. As witnessed by some of the newest
>CAP 'standards' (including the question in question...no pun
intended),
>e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens
>must be tested, and where 10 of the positives must be weakly positive
>-- an acknowledgment that validation specimens must be carefully
>selected in order to obtain appropriate results), it certainly
doesn't
>appear that the regulation of IHC testing is going to become more
relaxed.
>
>Joe Myers, M.S., CT(ASCP)
>
>------------------------------
>
>Message: 12
>Date: Fri, 18 Jun 2010 12:38:07 -0700
>From: "Thomas Jasper" <tjasper <@t> copc.net>
>Subject: RE: [Histonet] New CAP question ANP.22760
>To: "Mark Tarango" <marktarango <@t> gmail.com>
>Cc: _histonet <@t> lists.utsouthwestern.edu_
>(mailto:histonet <@t> lists.utsouthwestern.edu)
>
>Mark,
>
>Did you notice the credentials from this CAP representative? MT with
a
>Blood Bank specialty I believe. What I glean from that is...more
than
>likely this person does not grasp the logistics of
"contemporaneously"
>staining identical Abs from separate lots. She also likely does not
>understand the logistical application for detection and automation
>either.
>
>I'm not trying to be overly critical of this person. I'm sure she is
>quite intelligent and would not have the MT/SBB if she wasn't
>intelligent. It comes down to a lack of understanding Anatomic
>Pathology testing application re: automated IHC. I believe this is a
>common problem in and out of CAP. Many lab directors and other folks
in
>positions of authority without AP/Histology/Cytology backgrounds seem
>to believe that broad clinical lab modalities apply to Anatomic Path
>scenarios. I used to refer to this in my former position as -
"Trying
>to put the yoke of clinical lab onto anatomic path." We are
>laboratorians, but in many instances do not fit the general clinical
>lab mold.
>
>It's unfortunate that CAP has put this person in the position to
>respond. It is apparent to me that she's not grasping the
particulars
>here. She probably never will unless she decides to go into a
working,
>automated IHC "tissue" lab and take the time to ask questions and
>understand (learn) what we're all about.
>
>Thanks,
>Tom Jasper
>
>Thomas Jasper HT (ASCP) BAS
>Histology Supervisor
>Central Oregon Regional Pathology Services Bend, OR 97701
>_______________________________________________
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