[Histonet] PMS2

[Dana Settembre]

Diane,
When I was doing PMS2 I was using BD's mouse antibody @ 1:10
Retreived with Dako's TRS in a steamer for 40 min,
incubated the antibody for 60 minutes
and I used a labelled polymer for the detection (Dako's Envision +
Mouse)
It was difficult to work up.
Good Luck,


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> <DianaRip1 <@t> aol.com> 08/24/10 8:47 PM >>>
Can anyone share their protocol for PMS2?  I just keep getting 
background 
staining.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet