[Histonet] PMS2

Dana Settembre settembr <@t> umdnj.edu
Wed Aug 25 05:12:36 CDT 2010

When I was doing PMS2 I was using BD's mouse antibody @ 1:10
Retreived with Dako's TRS in a steamer for 40 min,
incubated the antibody for 60 minutes
and I used a labelled polymer for the detection (Dako's Envision +
It was difficult to work up.
Good Luck,

Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA

>>> <DianaRip1 <@t> aol.com> 08/24/10 8:47 PM >>>
Can anyone share their protocol for PMS2?  I just keep getting 
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