[Histonet] Fontana

Mike Pence mpence <@t> grhs.net
Wed Aug 18 12:19:39 CDT 2010

What stain platform are you using?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin,
Sent: Wednesday, August 18, 2010 11:04 AM
To: histonet
Subject: [Histonet] Fontana

Hello everone,
We recently stained about 20 slides with Fontana for a research project.
They were all fine except for 3 or 4 slides on which the pathologist
says that the stratum corneum is overstained.  The rest of the tissue is
fine but that layer is too black.  Now I have to fix the problem.  Does
anyone have an idea of why just that layer would overstain? On only a
few slides of the set?

Thank you!

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology

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