[Histonet] Fontana

[Martin, Erin]

Hello everone,
We recently stained about 20 slides with Fontana for a research project.  They were all fine except for 3 or 4 slides on which the pathologist says that the stratum corneum is overstained.  The rest of the tissue is fine but that layer is too black.  Now I have to fix the problem.  Does anyone have an idea of why just that layer would overstain? On only a few slides of the set?

Thank you!
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248