[Histonet] Sakura Tape coverslipper

Jackie M O'Connor Jackie.O'Connor <@t> abbott.com
Wed Aug 11 13:50:08 CDT 2010

OMG.   This problem was ever present in our lab for years, until I figured 
out that ANY minute trace of moisture, water, eosin in the last absolute 
alcohol would interfere with the activation of the adhesive in the tape, 
and create this brown artefact.   We religiously change our absolute 
alcohols on the autostainer so the last alcohol is perfectly clear of any 
hint of eosin, and the artefact has disappeared from our tape-coverslipped 
slides.    The Sakura rep couldn't help me either, and told me they had 
NEVER seen this problem before.   Love it.

Keep your last 100% PRISTINE, and the artefact will be eliminated.   I 

Jackie O'

"Nails, Felton" <flnails <@t> texaschildrens.org>
"'Gauch, Vicki'" <GauchV <@t> mail.amc.edu>, 
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
08/11/2010 01:45 PM
[Histonet] Sakura Tape coverslipper
Sent by:
histonet-bounces <@t> lists.utsouthwestern.edu

Has anyone been experiencing a brown cornflaking artifact on their slides 
after being coverslipped.
I was told by Sakura that it was do to the slides drying out when 
transferred from the stainer to the coverslipper.
Which can't be correct because on the new sakura coverslipper there is a 
holding area which is filled with xylene so if the tissue dried out it 
would rehydrate before being coverslipped. 

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