[Histonet] Re: Job Posting- Gainesville, Fl- Shands Hospital

Kendall Neely neelyk <@t> shands.ufl.edu
Wed Aug 11 12:43:30 CDT 2010

Position open at Shands Hospital in Gainesville, Fl
Histotechnologist, Full-time, day shift

Kendall A. Neely
Histology Technical Specialist
Shands Rocky Point Laboratories
(352) 265-0111, x72113

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Today's Topics:

   1. histological process video (Liette Tougas)
   2. brain microvessel staining (Niloofar Rezvani)
   3. Tissue Tek SCA Coverslipper (Mighnon Lashus)
   4. Paraffin (zodiac29 <@t> comcast.net)
   5. Slide label printers (Hannen, Valerie)


Message: 1
Date: Tue, 10 Aug 2010 13:51:19 -0400
From: Liette Tougas <ltougas <@t> dawsoncollege.qc.ca>
Subject: [Histonet] histological process video
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
	<7618BC6D53F39149B7B57615C218AADF114F5F35AC <@t> EXCHANGE.ad.dawsoncollege.qc.ca>
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Good day,

I am looking for a video/CD presentation of the whole histological process for the Histotechniques course.  Thank you very much in advance for a prompt reply,

Liette Tougas
Faculty, Medical Laboratory Technology Program
Dawson College, Montreal, Canada


Message: 2
Date: Tue, 10 Aug 2010 14:16:37 -0400
From: "Niloofar Rezvani" <Niloofar.Rezvani <@t> usuhs.mil>
Subject: [Histonet] brain microvessel staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4C615F64.B59E.002E.0 <@t> usuhs.mil>
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I appreciate if someone can answer my question. I am going to stain rats' brain microvessels with ER(estrogen receptor) antibody. We know how we can isolate the vessels but this is the first time to stain them.
I have the microvessels in PBS 1X and do you know how I can fix them with paraformaldehyde and mount them on the gel-subbed slides? I did 2 ways: 1- mount the fresh microvessels on the slide and wait overnight to get dried and then fix them in 2% PFA for 30 min and after that washing steps and the rest of IHC protocol or 2- fix the vessels with 2% PFA in the tube for 30 min and then mount them on the slides and wait overnight to get dried.
Please advise me with any idea,
thanks so much,

Dr. Niloofar Rezvani,Ph.D,PharmD
Postdoctoral Fellow
Graduate School of Nursing
Uniformed Services University
Email: niloofar.rezvani <@t> usuhs.mil 

Classification:  UNCLASSIFIED 
Caveats: None

Classification:  UNCLASSIFIED 
Caveats: None


Message: 3
Date: Tue, 10 Aug 2010 15:38:46 -0500
From: Mighnon Lashus <MLashus <@t> pathgroup.com>
Subject: [Histonet] Tissue Tek SCA Coverslipper
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
	<197CD0B02A81F94994A285C59C8AE05C05D29B9207 <@t> pgnexchange.pathgroup.com>
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I am having a problem with my tape coverslipper.  The coverslipper is not positioning the tape properly; when the slides are coverslipped, they have a 1mm overhang on the right side of the slide, would anyone know how to adjust this?

Mighnon Lashus, HT (ASCP)
PathGroup Lab
4071 S. Access Road, Suite 107
Chattanooga, TN  37406
423-493-0208 fax
mlashus <@t> pathgroup.com 

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Message: 4
Date: Wed, 11 Aug 2010 12:46:46 +0000 (UTC)
From: zodiac29 <@t> comcast.net 
Subject: [Histonet] Paraffin
To: histonet <@t> lists.utsouthwestern.edu 
	<1464661975.1354742.1281530806687.JavaMail.root <@t> sz0062a.westchester.pa.mail.comcast.net>
Content-Type: text/plain; charset=utf-8


Just wanted to know if anyone has tried Henry's Wonder Wax Xtreme Paraffin w/DMSO 
made by Mercedes Medical. What were your expirences with it? Is it comprable to McCormick's Paraplast Xtra? 

Thanks in advance, 



Message: 5
Date: Wed, 11 Aug 2010 09:54:39 -0400
From: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>
Subject: [Histonet] Slide label printers
To: <histonet <@t> lists.utsouthwestern.edu>
	<5680DA93771F0C48954CC8D38425E72401AB3512 <@t> ISMAIL.parrishmed.local>
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Hi gang...
I am hoping that someone out there can lend me some assistance. We
currently are using paper slide labels( 0.875 in by 0.875 in) on our
Pathology slides, an old label printer (Genicom Model 1025) and Meditech
Magic software.  Our dilemma is this... we no longer can find the ink
ribbons for our printer. Is there anyone out there who is using the same
In about 1 1/2 to 2 years, we are going to be changing to a new software
(possibly McKesson), so we are not looking to really buy anything
expensive at this time. We just really need to continue using the paper
labels until we have the change over.
Thanks for your help.
Valerie Hannen, MLT (ASCP),HTL,SU(FL)
Parrish Medical Center

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