[Histonet] Precipitate in Processor

Jon St.Onge Jon.St.Onge <@t> dako.com
Mon Aug 9 11:23:22 CDT 2010

That is a phosphate precipitate most likely from your 10%NBF.
Try going into a 70% ETOH first followed by higher concentrations.

To see for yourself pour 10%NBF into graded alcohols- Precipitate is easy to see with 100% ETOH

Jon Henry St. Onge
Dako North America
Quality Control Supervisor

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adrienne Aperghis Kavanagh
Sent: Monday, August 09, 2010 9:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Precipitate in Processor

Hello Everyone,  

Has anyone ever seen a (salt?) precipitate in their alcohols following formalin?  While changing the processor this morning, I noticed a precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% alcohol).  It is white and grainy.  The alcohols were otherwise unaffected.

We are using a 10% NBF containing:
Sodium Phosphate, monobasic
Sodium Phosphate, dibasic

And our alcohols are all reagent grade.

Any help would be very much appreciated!  Thank you in advance!

Adrienne Aperghis Kavanagh 
30 W. Century Road 
Suite 255 
Paramus NJ 07652 

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