[Histonet] friable or crumbly O.C.T.

jsjurczak <@t> comcast.net jsjurczak <@t> comcast.net
Mon Aug 9 09:49:11 CDT 2010

There is an OCT for lower temperatures. 
----- Original Message ----- 
From: "Della Speranza, Vinnie" <dellav <@t> musc.edu> 
To: "Bruce W Brodersen" <bbroders <@t> unlnotes.unl.edu>, histonet <@t> pathology.swmed.edu 
Sent: Monday, August 9, 2010 9:40:03 AM 
Subject: RE: [Histonet] friable or crumbly O.C.T. 

I'm guessing that liquid nitrogen or dry ice temperature is too cold for sectioning OCT. 
OCT cuts well down to about -25 degrees C. 
Liquid Nitrogen is about -160 degrees C. I believe dry ice is in the same temp range at LN2 

You will want to give the OCT blocks the opportunity to "warm up" to cryostat temperature before attempting to section them. Leave your frozen blocks in the cryostat for 30-60 minutes before sectioning to allow them to come to optimum temperature 

Vinnie Della Speranza 
Manager for Anatomic Pathology Services 
Medical University of South Carolina 
165 Ashley Avenue  Suite 309 
Charleston, South Carolina 29425 
Tel: (843) 792-6353 
Fax: (843) 792-8974 

-----Original Message----- 
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bruce W Brodersen 
Sent: Friday, August 06, 2010 2:26 PM 
To: histonet <@t> pathology.swmed.edu 
Subject: [Histonet] friable or crumbly O.C.T. 

Anyone have an explanation as to why OCT would be friable or crumbly for 
sectioning?  Here's how it was used. 

"We held the plastic 'tray' with the tissue in the compound just over the 
liquid nitro for 30sec-1min, until it was opaque and white (frozen) and 
then dipped the tray into the liquid nitro for 20-30sec., placed in small 
bags and then into a cooler with dry ice until shipping." 

Bruce W. Brodersen, DVM, PhD 
University of Nebraska Veterinary Diagnostic Center 
1900 N. 42nd Street 
Lincoln, NE  68583-0907 

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