[Histonet] de-wax without organics

Sebree Linda A LSebree <@t> uwhealth.org
Fri Aug 6 14:37:34 CDT 2010


This is exactly how our Ventana instruments deparaffinize our slides and
we've never had a problem regarding this methodology. 

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Goins,
Sent: Friday, August 06, 2010 2:13 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] de-wax without organics

We have run some preliminary tests on de-waxing slides using warm
detergent solutions rather than xylene or xylene substitutes.  So far
the pathologists see no difference between the H&E stained slides
treated with detergent vs. the usual xylene to alcohol to water route;
the cellular detail is equivalent and the dye reactions appear
identical.   We have also run comparisons for several special stains and
IHC slides with good results.

I would greatly appreciate hearing from anyone who is aware of reasons
why this is a bad idea!  It is quicker and reduces the volume of
organics required by half so we are seriously considering it as an
alternative protocol.

Thanks in advance for your feedback.


Tresa Goins
Veterinary Diagnostic Lab
Department of Livestock
Bozeman, Montana

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