[Histonet] Ventana IHC/ recycled reagents

[Sebree Linda A]

Melissa,

First, I would make sure that when you create your control slides that
you only dip the top end of the slide into your water bath to pick up
your control tissue section thus the bottom part of the slide where the
patient tissue will go has never been wet or collected debris from your
water bath.

Second, I would pick up your sections with core/needle biopsies so that
the tissue runs parallel to the long edges of the slide.  Your wash
buffers will encounter less resistance from the tissue this way and
hopefully not so readily dislodge your tissue.

Just a couple thoughts that might help.


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kuhnla,
Melissa
Sent: Friday, August 06, 2010 5:03 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana IHC/ recycled reagents

Anyone running IHC on Ventana platforms? Do you run patient samples on
the same slide as control tissue? Do you have problems with sections
adhering to the slides?  The largest culprit for us seems to be
small/fine needle core biopsies.  Any insight into why this shape is
problematic?  Over-processed maybe?

Is anyone using recycled alcohols or Xylene in their tissue processors?
Do you find this to be problematic?  

Thank you,
Melissa
Lead Med. Technologist, IHC/ISH
Catholic Health Services of Long Island


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