[Histonet] Ventana IHC/ recycled reagents

[BSullivan <@t> shorememorial.org]

We try to run both the positive control and patient tissue on the same
slide when size allows this. There are times, especially with cell blocks
that we have problems with tissue loss. We have started to mount all cell
blocks on their own slide. One thing that we have implemented and seems to
work great is making sure that all debris, and this includes melted
paraffin from the control, be removed from the control slide. A clean slide
is important for adherence of the tissue.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


                                                                           
             "Kuhnla, Melissa"                                             
             <Melissa.Kuhnla <@t> c                                             
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                                       [Histonet] Ventana IHC/ recycled    
                                       reagents                            
             08/06/2010 06:02                                              
             AM                                                            
                                                                           
                                                                           
                                                                           
                                                                           




Anyone running IHC on Ventana platforms? Do you run patient samples on
the same slide as control tissue? Do you have problems with sections
adhering to the slides?  The largest culprit for us seems to be
small/fine needle core biopsies.  Any insight into why this shape is
problematic?  Over-processed maybe?

Is anyone using recycled alcohols or Xylene in their tissue processors?
Do you find this to be problematic?

Thank you,
Melissa
Lead Med. Technologist, IHC/ISH
Catholic Health Services of Long Island


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