[Histonet] Pen Slides for LCD

[Orr, Rebecca]

Hi Mary,
We had a similar issue. I was  asked to troubleshoot this problem for our researchers...and I didn't make a copy of the literature and gave it away....or I'd quote directly...

You'll have to call Leica and get the specific tech support area that  handles LCD.  They have a "memo" that she sent me that describes curing the membrane slides under UV for a specific amount of time (I think 1/2 hour) before picking up tissue.
The product insert that comes with the slides do not address this.
Once we did the UV step it was fine, tissue did not wash off.
She was very nice and very knowledgeable and sent me the memo no probs.

Hope this helps.


Becky Orr CLA,HT(ASCP)QIHC
Technical Specialist
Anatomic Pathology
NorthShore University HealthSystem
847-570-2771




Message: 14
Date: Thu, 5 Aug 2010 12:51:48 -0400
From: "Mary Lloyd" <lloyd.3 <@t> osu.edu>
Subject: [Histonet] PEN SLIDES FOR LASER CAPTURE
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Does anyone have any experience with PEN membrane slides from Zeiss for
laser capture microdissection.  I have been working with one of our
residents and we are having problems with the tissue staying on these
slides. We could sure use help.  Thanks Mary Lloyd