[Histonet] TUNEL of frozen thick rat brain slices.

Wed Aug 4 11:21:15 CDT 2010

Dear Histologists,
Has anyone done TUNEL on 40 um flash-frozen rat brain sections? I have tried tweaking the protocol (we use the Millipore Apoptag kit  - S7101) from our ongoing 20 um slices by increasing the incubation times from 1 hour to 2 and 4 hours but we still get poor staining. Also, could I do overnight incubations? the tissue is flash frozen, but it does get fixed at the beginning of the protocol with PFA (4%) and subsequently with acetic acid-EtOH.
Thanks,
Gil

-- 
Guillermo Palchik
Ph.D. Candidate - Interdisciplinary Program in Neuroscience
Georgetown University Medical Center
Research Building Room W 217
3970 Reservoir Rd. NW, Washington, DC 20007
Lab: 202-687-7825