[Histonet] Olig2 (ab33427) on the bond max

Houston, Ronald Ronald.Houston <@t> nationwidechildrens.org
Wed Aug 4 08:07:11 CDT 2010


I had problems with this one too. It now seems that this particular antibody has been removed from AbCam's product range due to some QC problems.

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Malika Benatti
Sent: Wednesday, August 04, 2010 6:14 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Olig2 (ab33427) on the bond max

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** Reply Requested When Convenient **

I was wondering if anyone on the histonet list is using abCam Olig2 (ab33427) on the bond max.
I have been trying various titration and enzyme treatment on surgical brain tissue with not much success al we seams to achieve is nuclear staining and heavy background staining. 

Any suggestions would be appreciated! 

Thanks in advance 

Malika


 


Malika Benatti
 
Specialist BMS 
Camelia Botnar Laboratories
Histopathology Department
Great Ormond Street Hospital
London WC1N 3JH

 
Tel:  +44 20 7405 9200 ext 5475
Fax:  +44 20 7829 7875
 
benatm <@t> gosh.nhs.uk


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