[Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ?

Wed Apr 28 10:13:35 CDT 2010

Michael,

Thanks for clarifying that.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net

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From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of
Michael Ho
Sent: Wednesday, April 28, 2010 9:04 AM
To: Van Eyck, Deb; ancillarypath <@t> mac.com; ihcrg Group (E-mail); histonet
netserver
Subject: RE: [IHCRG] ER clone 1D5 or SP1 ?

In response to Debs post the other day I would like  clarify that the  PR
antibody  clone 312  cited in the new ASCO/CAP guidelines is in fact a
product number for the Novocastra clone 16.

Michael Ho

Resource Technologist

Immunopathology

Division of Pathology

DPLM

The Hospital for Sick Children

555, University Avenue

Toronto, Ontario, M5G1X8

Canada

Tel# 416-813-5950

FAX#416-813-5974

From: Van Eyck, Deb [mailto:deb.vaneyck <@t> phci.org] 
Sent: Monday, April 26, 2010 6:12 PM
To: Michael Ho; ancillarypath <@t> mac.com; ihcrg Group (E-mail); histonet
netserver
Subject: RE: [IHCRG] ER clone 1D5 or SP1 ?

Thanks Michael ----I saw table 10 -but on page 16 they specifically only
list clone 1294 and 312 (which is whose?) -they really don't clearly say all
clones for PR listed in table 10.  They also only refer to these two again
on the shortened ASCO color plate handout Additional information in re:
clinical question 2.1.  Deb

  _____  

From: Michael Ho [mailto:michael.ho <@t> sickkids.ca] 
Sent: Monday, April 26, 2010 4:13 PM
To: Van Eyck, Deb; ancillarypath <@t> mac.com; ihcrg Group (E-mail); histonet
netserver
Subject: RE: [IHCRG] ER clone 1D5 or SP1 ?

Hi Deb

If you look at table 10 of the new guidelines you will see the ER/PR
antibody clone list

Michael

  _____  

From: ihcrg <@t> googlegroups.com [ihcrg <@t> googlegroups.com] On Behalf Of Van Eyck,
Deb [deb.vaneyck <@t> phci.org]
Sent: April 26, 2010 12:29 PM
To: ancillarypath <@t> mac.com; ihcrg Group (E-mail); histonet netserver
Subject: RE: [IHCRG] ER clone 1D5 or SP1 ?

This is a great discussion lets also talk about PR clones since the ASCO/CAP
guidelines just came out ------Hadi or Rich I know they only list two PR
clones one is Dako 1294-----what is the other 312? Deb

  _____  

From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of
ancillarypath <@t> mac.com
Sent: Sunday, April 25, 2010 7:36 PM
To: ihcrg Group (E-mail); histonet netserver
Subject: Re: [IHCRG] ER clone 1D5 or SP1 ?

When we started our lab 3 years ago, we began with SP1 from day 1, so I
don't have any experience with either 1D5 or 6F11 except in my previous
labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in
the work-up of metastatic carcinoma of unknown primary site, based on the
published literature. The advantage of 6F11 is that, for those of us who use
the Allred scoring system, it's the only clone that was clinically validated
by Harvey et al. (JCO 1999) for this purpose. I agree with Rich.

For those who use SP1, it's a very good clone as a predictive marker in
breast cancer. But again, in the setting of metastatic workup, it is NOT
recommended, as it will pick up too many primary lung cancers and some colon
cancers (personal experience).

Hadi

================================

Hadi Yaziji, M.D., Medical Director

Vitro Molecular Laboratories

President,

Ancillary Pathways

7000 62nd Avenue, PH-C

Miami, FL 33143

T 305-740-4440

F. 786-513-0175

www.vitromolecular.com

www.ancillarypath.com

On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote:

I have looked at several clones over the years and I prefer clone 6F11.

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

"Taylor, Jean" <jtaylor <@t> meriter.com> 4/23/2010 11:17 AM >>>

I'm wondering which clone of ER most labs are using?

Thanks,
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI

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