[Histonet] LR white sections

[Prior, Andrew]

Fellow histonetters, 
I'm looking for advice for cutting bone samples embedded in LR white resin on a microtome.
We have plenty of experience cutting decalcified bone in wax and with sawing/grinding sections, but little experience in cutting thin sections of resin embedded bone.
We will be using a Leica rotary microtome and tungsten carbide blades, aiming to cut at 5µm thickness. 
I am looking for advice on infiltration times, embedding, sectioning and staining. Any and all advice gratefully received.
Many thanks

Andrew

Andrew Prior
Histologist
Smith &Nephew Research Centre
York
YO10 5DF
UK
 Andrew.Prior <@t> smith-nephew.com 
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