[Histonet] Mysterious artifact on GI biopsies H&E stain

[Mike Pence]

It could be a "hot biopsy"?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Urim,
Lyudmila
Sent: Monday, April 26, 2010 6:24 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain




> Hi,
> 
> We have been getting a similar staining artifact on some GI biopsies 
> randomly while the rest GI biopsies look fine.  Also on the affected 
> GI biopsies there is often only 1 or 2 spots of the biopsy that are 
> affected while the rest of the biopsy looks fine. The artifact is 
> being reproduced on each cut; also the artifact doesn't disappear 
> after the affected biopsies get reprocessed. The artifact can be 
> described as a foggy, smudgy stain, with deformed nucleus and not 
> enough cell details. All our specimens are fixed in 10%NBF.
> 
> Please let me know if you have any ideas.
> Lucy
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