[Histonet] Mysterious artifact on GI biopsies H&E stain

Urim, Lyudmila lurim <@t> challiance.org
Mon Apr 26 06:23:57 CDT 2010



> Hi,
> 
> We have been getting a similar staining artifact on some GI biopsies
> randomly while the rest GI biopsies look fine.
>  Also on the affected GI biopsies there is often only 1 or 2 spots of
> the biopsy that are affected while the rest of the biopsy looks fine. 
> The artifact is being reproduced on each cut; also the artifact
> doesn't disappear after the affected biopsies get reprocessed.
> The artifact can be described as a foggy, smudgy stain, with deformed
> nucleus and not enough cell details. 
> All our specimens are fixed in 10%NBF.
> 
> Please let me know if you have any ideas.
> Lucy


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