[Histonet] Gram stain

[Maxim Peshkov]

Margaret:
We uses 1% aqueous neutral red as red counterstain
with very good results.
Do not forget rinse slides at 10-15 secs in DW
with 1-2 drop of glacial acetic acid before neutral
red and after this, because this dye have red color
at pH < 6.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.

---Original message---
> Date: Fri, 23 Apr 2010 16:25:30 -0500
> From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
> Subject: [Histonet] Gram stain
> To: "histonet <@t> lists.utsouthwestern.edu"
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> <FCA5EF47F9BC694CBB4C58FEA04219634CCFB69B74 <@t> SDSU-MBX.jacks.local>
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> Now that I'm done with my rant I have a real
> question.  We are trying to do a gram stain on fish
> and the safranine O is staining everything red.  What
> other stain would you use?  I usually have time to
> look at the books but unfortunately it's almost
> quiting time and the slides need to be done by noon Monday.  Thanks
> Margaret Perry

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