[Histonet] IHC Slides, Hotplate vs Oven

[Tony Henwood]

We use an oven (62oC) for 25min only. See:

Henwood, A., (2005) "Effect of Slide Drying at 80°C on Immunohistochemistry" J Histotechnol 28(1):45-46.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Wednesday, 21 April 2010 12:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Slides, Hotplate vs Oven


I'm curious as to whether or not others put their IHC slides on a hotplate after cutting?  If so, at what temperature and for how long? Who only uses an oven - and what temp and for how long?  Does anyone use both the hotplate and the oven?

 

Laurie Colbert

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