[Histonet] IHC Slides, Hotplate vs Oven

[Sebree Linda A]

I sometimes put cut slides on a warm plate attached to my water bath.
One of the plates is set at 55d and the other at 60d.  I leave them till
all the paraffin is completely melted.   I only do this for cases I'm
"paranoid" about staying on! 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Tuesday, April 20, 2010 9:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Slides, Hotplate vs Oven

I'm curious as to whether or not others put their IHC slides on a
hotplate after cutting?  If so, at what temperature and for how long?
Who only uses an oven - and what temp and for how long?  Does anyone use
both the hotplate and the oven?

 

Laurie Colbert

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