[Histonet] retrieveing DNA from a mounted H&E slide

[louise renton]

Long, long ago when dinosaurs roamed and PCR was an emerging technique, what
I would do is as follows:

REmove coverslip in appropriate solvent, soaking te get any mounting media
residue off. Rinse slide in abs alc and the 70% alcohol and then scrape off
tissue into an Eppy with a new disposable blade. (Obviously, wearing gloves
and taking care not to cut yourself). Rinse in sterile water, spin down, pur
off supernatant , and contine as for normal extraction with your usual lysis
buffer/extraction system. (back then we were using phenol chloroform!)

The quality of the DNA is dependent on one of several factors, including the
fixation medium, length of fixation, section thickness etc.



On Mon, Apr 19, 2010 at 7:40 PM, Barone, Carol <cbarone <@t> nemours.org> wrote:

> Hello histonetter's...I come one again to the experts in the field.
>
> I have been give two H&E slides from an autopsy case (have no idea how
> old)...and have been asked to retieve this tissue into an eppy with
> lysis buffer for DNA. Does anyone out there have an existing method for
> doing this? I have a notion what is needed to do this (though I am
> somewhat skeptical on the quality of the DNA retieved from the tissue)
> .....but, perfer not to re-invent the wheel...So if someone out there
> has a method for doing this, I would certainly appreciate it, if you
> wouldn't mind sharing it with us. Thanks... to all! CB
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-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
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