[Histonet] phosphoantibodies

MKing making <@t> ufl.edu
Mon Apr 19 09:24:38 CDT 2010


Ashley,
Phosphatases are relatively resistant to fixation which is why the 
histochemical phosphatase methods work.  You may want to try putting 
phosphatase inhibitors in you fix or post-fix solutions to stop 
dephosphorylation, and see if it makes a difference in your ihc.  I 
think Sigma has a table of reagents somewhere on their website and I'll 
email the link if I can find it.
Good luck,
Mike
UF Pharmacology & Therapeutics

------------------------
Date: Fri, 16 Apr 2010 14:43:15 -0500
From: "Troutman, Kenneth A" <kenneth.a.troutman <@t> Vanderbilt.Edu>
Subject: [Histonet] Phospho antibodies and fixation
To: "Histonet <@t> lists.utsouthwestern.edu"
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:<7B310892042DA74CB3590053F424CFE60B7480EFBC <@t> ITS-HCWNEM06.ds.Vanderbilt.edu>
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Does anyone have any information on phospho antibodies and fixation?...
Thanks,
Ashley Troutman BS, HT(ASCP) QIHC
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4532
Nashville, TN  37232
615-343-9134



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