[Histonet] phosphoantibodies

[MKing]

Ashley,
Phosphatases are relatively resistant to fixation which is why the 
histochemical phosphatase methods work.  You may want to try putting 
phosphatase inhibitors in you fix or post-fix solutions to stop 
dephosphorylation, and see if it makes a difference in your ihc.  I 
think Sigma has a table of reagents somewhere on their website and I'll 
email the link if I can find it.
Good luck,
Mike
UF Pharmacology & Therapeutics

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Date: Fri, 16 Apr 2010 14:43:15 -0500
From: "Troutman, Kenneth A" <kenneth.a.troutman <@t> Vanderbilt.Edu>
Subject: [Histonet] Phospho antibodies and fixation
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Does anyone have any information on phospho antibodies and fixation?...
Thanks,
Ashley Troutman BS, HT(ASCP) QIHC
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4532
Nashville, TN  37232
615-343-9134