[Histonet] hematoxylin-eosin saffron

[Bryan Hewlett]

Kris,

It is a general oversight stain as is the H&E.
It is correctly refered to as the Masson Hematoxylin-Phloxine-Saffron (HPS) 
stain.
Although some people substitute eosin for phloxine, this is a generally 
inferior technique to HPS (I have NEVER seen a good one!).
Technically, the method is harder to control than the H&E or a separate 
Trichrome, and Saffron is much more expensive than other suitable yellow 
substitutes.
However, those labs that still use the method like it because it has many of 
the attributes of a trichrome stain.

Bryan

----- Original Message ----- 
From: "Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, April 16, 2010 1:44 PM
Subject: [Histonet] hematoxylin-eosin saffron


Can someone please explain to me the purpose of a hematoxylin-eosin
saffron stain.  Why would this be used over the normal hematoxylin and
eaosin stain.  Thank you in advance.

Kris
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