[Histonet] ms<pig cd31

[Merced M Leiker]

Hi Patsy,

I'd like to verify the retrieval temp? In a previous email you wrote "70dc" 
and here you wrote "80dc."

Thank you.

Regards,
Merced

--On Wednesday, April 14, 2010 10:54 AM -0600 Patsy Ruegg 
<pruegg <@t> ihctech.net> wrote:

> For all those looking for this info from me.
>
>
>
> I use the serotech mouse anti porcine CD31 on pig tonsil at 1/10-1/50
> overnight after HIER in a waterbath set at 80dc for 3 hours with prewarmed
> citrate buffer ph6.  I use this with an anti mouse labeled polymer
> detection system, either hrp/dab or alk.phos./red.  You can join the
> group online at www.ihcrg.org
>
>
>
> This one is tuff, but this is what worked for us.
>
>
>
> Patsy
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email pruegg <@t> ihctech.net
> web site www.ihctech.net
>
>
>
>
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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