[Histonet] ms<pig cd31

[Patsy Ruegg]

For all those looking for this info from me.

 

I use the serotech mouse anti porcine CD31 on pig tonsil at 1/10-1/50
overnight after HIER in a waterbath set at 80dc for 3 hours with prewarmed
citrate buffer ph6.  I use this with an anti mouse labeled polymer detection
system, either hrp/dab or alk.phos./red.  You can join the group online at
www.ihcrg.org 

 

This one is tuff, but this is what worked for us.

 

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net

 


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