[Histonet] IHC and IF
Thotakura, Anil Kumar
a.thotakura <@t> imperial.ac.uk
Thu Apr 15 06:14:26 CDT 2010
Dear All,
I have kind of funny problem.
I am doing IHC (substrate reaction suing Dabs reagent) and IF ( direct
labeling of my monoclonal antibody with flurochrome).
I am working on mouse liver frozen sections. When I did IHC the staining
looks like cytoplasm and if I do IF for the same protein I saw nuclear
staining. I am unable to conclude whether it is cytoplasm or nuclei
staining.
Please advice. The monoclonal antibody I used is not commercially available.
We made it.
Thanks In advance.
Many Thanks,
Anil Kumar.
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