[Histonet] Vibrating microtome sections

[Mike Tighe]

I would like cut fresh spleen and lymph node sections on a vibrating microtome. I have tried embedding in low melt agarose but the tissue seems to be too flexable to get good repeating sections (uniform thickness). When the knife does catch the cells seem to pour out into the surrounding medium. Does anybody have a suggestion or a reliable method they would be willing to share? Maybe there is a embedding medium that I am not aware of?

Thanks for any Help!!
Mike