SPAM-LOW: [Histonet] Re: Cd31 in pigs

[Patsy Ruegg]

A few years ago I spent a lot of effort on using Serotec's mouse
anti-porcine CD31 for ffpe porcine tissue and I did get some positive
results with unusual pretreatment methods, as I recall I had to use a
waterbath for hier set at 70dc for 3 hours in hph buffer.  I will look up
the method at work on Monday and send it to you if you contact me again to
remind me.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Wilson
Sent: Friday, April 09, 2010 1:44 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] Re: Cd31 in pigs


Serotec's mouse anti-porcine CD31 (MCA1746) will work well in fresh, frozen,
acetone-fixed pig tissues. It is very good for angiogenesis. It does not
work with FFPE tissues.

Liz Wilson
Bethyl Labs
------------------------------

Message: 4
Date: Fri, 9 Apr 2010 11:59:44 -0400
From: "Jean-Martin Lapointe" <jm.lapointe <@t> accellab.com>
Subject: [Histonet] Cd31 in pigs
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BEFD613BD39142499989F836556DDC8394A809 <@t> ACE.accellab.lan>
Content-Type: text/plain;	charset="iso-8859-1"

Hi Joel,
we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had
no luck. We currently use an antibody to vWF to stain pig endothelium. If
someone has a working technique for CD31 in swine, I'd be happy to hear
about it.

Jean-Martin
__________________________________
Jean-Martin Lapointe, DMV, MS, dACVP
AccelLAB Inc
Quibec, Canada  J7H 1N8
jm.lapointe <@t> accellab.com

 
 
Date: Fri, 9 Apr 2010 09:40:00 -0400
From: "Joel Israel" <JoelI <@t> mcclainlab.com>

 
Does anyone have a procedure that WORKS for cd31 in pigs?  I can't seem
to get it to work no matter what I do.  Any help will be greatly
appreciated.  Thanks.- joel






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