[Histonet] Re: Cd31 in pigs

[Liz Wilson]

Serotec's mouse anti-porcine CD31 (MCA1746) will work well in fresh, frozen,
acetone-fixed pig tissues. It is very good for angiogenesis. It does not
work with FFPE tissues.

Liz Wilson
Bethyl Labs
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Message: 4
Date: Fri, 9 Apr 2010 11:59:44 -0400
From: "Jean-Martin Lapointe" <jm.lapointe <@t> accellab.com>
Subject: [Histonet] Cd31 in pigs
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BEFD613BD39142499989F836556DDC8394A809 <@t> ACE.accellab.lan>
Content-Type: text/plain;	charset="iso-8859-1"

Hi Joel,
we have tried a few antibodies for CD31 in FFPE tissues in pigs and have had
no luck. We currently use an antibody to vWF to stain pig endothelium. If
someone has a working technique for CD31 in swine, I'd be happy to hear
about it.

Jean-Martin
__________________________________
Jean-Martin Lapointe, DMV, MS, dACVP
AccelLAB Inc
Quibec, Canada  J7H 1N8
jm.lapointe <@t> accellab.com

 
 
Date: Fri, 9 Apr 2010 09:40:00 -0400
From: "Joel Israel" <JoelI <@t> mcclainlab.com>

 
Does anyone have a procedure that WORKS for cd31 in pigs?  I can't seem
to get it to work no matter what I do.  Any help will be greatly
appreciated.  Thanks.- joel