[Histonet] Re: Uranyl Nitrate
David Hill
dhill32 <@t> comcast.net
Wed Apr 7 19:28:58 CDT 2010
We use Protocol zinc formalin and it works great. Our steiners look better
than they ever had with uranyl nitrate. It's much cheaper also.
David Hill B.S. CT(ASCP)HTL,QIHC
Assistant Histology Supervisor
Trumbull Labs
Germantown, TN
----- Original Message -----
From: <histonet-request <@t> lists.utsouthwestern.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, April 07, 2010 12:02 PM
Subject: Histonet Digest, Vol 77, Issue 8
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> Today's Topics:
>
> 1. Re: Time allotted for Frozen sections (Rene J Buesa)
> 2. Double staining of the same Primary (Reuel Cornelia)
> 3. Uranyl Nitrate (Dawn Oakes)
> 4. RE: SPAM-LOW: [Histonet] qihc (Patsy Ruegg)
> 5. RE: anti-F4/80 on IntelliFlex (Patsy Ruegg)
> 6. Re: Uranyl Nitrate (Rene J Buesa)
> 7. amyloid ? for Bob Richmond (Carrie Disbrow)
> 8. TX Society meeting? (Cheryl)
> 9. Re: amyloid ? for Bob Richmond (Richard Cartun)
> 10. Re: Cleaning VIP Processor containers (Joseph Saby)
> 11. Re: Uranyl Nitrate (John Kiernan)
> 12. Cleaning VIP Processor Containers - Summary (Brandi Higgins)
> 13. Aniline blue (Itai Moshe)
> 14. Re: Uranyl Nitrate (Rene J Buesa)
> 15. picro sirius red, sirius red connective tissue
> stain[Scanned] (Itai Moshe)
> 16. frozen lung sections (Molinari, Betsy)
> 17. Old Manuals (Michael J. Lyon, Ph.D.)
> 18. RE: frozen lung sections (Dishop, Megan)
> 19. herring sperm dna (Emily Sours)
> 20. RE: Time allotted for Frozen sections (Kathy M. Gorham)
> 21. abtibodies (Cynthia Pyse)
> 22. instrument disinfection (Margiotta, Michele)
> 23. (no subject) (Santiago, Albert)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 6 Apr 2010 10:09:27 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Time allotted for Frozen sections
> To: histonet <@t> lists.utsouthwestern.edu, "Kathy M. Gorham"
> <KMB01 <@t> grh.org>
> Message-ID: <382702.38475.qm <@t> web65704.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> CAP requires an explanation as to why any FS diagnosis takes more than 20
> minutes after the sample reaches the lab.
> The average time is 15 minutes.
> René J.
>
> --- On Tue, 4/6/10, Kathy M. Gorham <KMB01 <@t> grh.org> wrote:
>
>
> From: Kathy M. Gorham <KMB01 <@t> grh.org>
> Subject: [Histonet] Time allotted for Frozen sections
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, April 6, 2010, 12:33 PM
>
>
> Good morning histoland. What is the maximum time allotted for frozen
> section per block? Is this written in CAP regs? JCAHO regs?
> Thanks, Kathy Gorham H.T.
>
>
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> ------------------------------
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> Message: 2
> Date: Tue, 06 Apr 2010 13:09:32 -0500
> From: "Reuel Cornelia" <Reuel.Cornelia <@t> tsrh.org>
> Subject: [Histonet] Double staining of the same Primary
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4BBB328C020000C500074CD7 <@t> mail.TSRH.ORG>
> Content-Type: text/plain; charset=US-ASCII
>
> Can anybody share their protocol for Rat spinal cord double immunostain
> both flourescent and ABC-HRP of the same species (mouse) Primary antibody.
> Thank you.
>
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 6 Apr 2010 11:49:05 -0700
> From: Dawn Oakes <doakes <@t> olympicmedical.org>
> Subject: [Histonet] Uranyl Nitrate
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <F5ECF3459F04CB43850D1EAC8180A737C3E683 <@t> is-210vs.olympicmedical.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Is there any substitutes for Uranyl Nitrate in the modified Steiner
> Stain? How is it being disposed of?
>
>
>
> Dawn Oakes HT
>
> Olympic Medical Center
>
>
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> ------------------------------
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> Message: 4
> Date: Tue, 6 Apr 2010 14:56:52 -0600
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: RE: SPAM-LOW: [Histonet] qihc
> To: "'Sara Baldwin/mhhcc.org'" <sbaldwin <@t> mhhcc.org>, "'histonet'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <CD8FE87D309E49FD809F7A03F7540FA8 <@t> prueggihctechlt>
> Content-Type: text/plain; charset="us-ascii"
>
> I do not believe so. There are some questions from the Self Assessment
> booklet from NSH on IHC/EM and Enzyme Histochemistry but since it has so
> many subjects to cover not much space left for just IHC. Your best bet is
> the Dako Handbook on IHC and the references listed on the IHC Resource
> Group
> website, www.ihcrg.org , join the group online from the website to access
> the references and the pp presentation on Taking the QIHC by Ethel Macrea,
> gotta be a member of NSH to join the IHCRG but it is a great resource.
>
> Regards,
>
> Patsy
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sara
> Baldwin/mhhcc.org
> Sent: Tuesday, April 06, 2010 8:32 AM
> To: histonet
> Subject: SPAM-LOW: [Histonet] qihc
>
>
> Is there a practice test out there for the QIHC?
> ThanksPatholgy Supervisor
> Kathy Baldwin, SCT (ASCP)
> M=morial Hospital and Health Care Center
> [1]sbaldwin <@t> mhhcc.org
> Ph& nbsp;812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897
>
> Confidential information, Authorized use only.
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> References
>
> 1. ="mailto:sbaldwin <@t> mhhcc.org"
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>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 6 Apr 2010 14:59:30 -0600
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: RE: [Histonet] anti-F4/80 on IntelliFlex
> To: "'Connolly, Brett M'" <brett_connolly <@t> merck.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <3790E77523054A24B4B53F508B3DB815 <@t> prueggihctechlt>
> Content-Type: text/plain; charset="us-ascii"
>
> Brett,
>
> I run F4/80 using the BC rat on ms detection (not their instrument)
> without
> getting BG issues, are you using Proteinase K digestion? I found that to
> work better than HIER.
>
> Patsy
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Connolly,
> Brett M
> Sent: Tuesday, April 06, 2010 9:03 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] anti-F4/80 on IntelliFlex
>
> Is anyone running the Serotec rat anti-mouse F4/80 on the Biocare
> IntelliFlex platform? We are having background problems.
>
> Thanks,
>
> Brett M. Connolly, Ph.D.
> Molecular Imaging Team Leader
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> tel. 215-652-2501 fax. 215-993-6803
> brett_connolly <@t> merck.com
>
>
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> ------------------------------
>
> Message: 6
> Date: Tue, 6 Apr 2010 14:01:53 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Uranyl Nitrate
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, Dawn Oakes
> <doakes <@t> olympicmedical.org>
> Message-ID: <653634.88459.qm <@t> web65707.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Yes, 1% aq. sol. of phosphotungstic acid.
> Want the reference?
> René J.
>
> --- On Tue, 4/6/10, Dawn Oakes <doakes <@t> olympicmedical.org> wrote:
>
>
> From: Dawn Oakes <doakes <@t> olympicmedical.org>
> Subject: [Histonet] Uranyl Nitrate
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Date: Tuesday, April 6, 2010, 2:49 PM
>
>
> Is there any substitutes for Uranyl Nitrate in the modified Steiner
> Stain? How is it being disposed of?
>
>
>
> Dawn Oakes HT
>
> Olympic Medical Center
>
>
> -------------------------------------------------------------------------
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>
> ------------------------------
>
> Message: 7
> Date: Tue, 06 Apr 2010 17:05:58 -0400
> From: "Carrie Disbrow" <disbrc <@t> shands.ufl.edu>
> Subject: [Histonet] amyloid ? for Bob Richmond
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4BBB69F6.72AC.0059.1 <@t> shands.ufl.edu>
> Content-Type: text/plain; charset=US-ASCII
>
>
>
> Hi,
> I was also told or read somewhere that insulinomas are good amyloid
> controls. I've never had a chance to test any blocks from these cases.
> What has your experience been? Thanks for your help.
> Carrie
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 6 Apr 2010 14:09:08 -0700 (PDT)
> From: Cheryl <tkngflght <@t> yahoo.com>
> Subject: [Histonet] TX Society meeting?
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <833406.16237.qm <@t> web50906.mail.re2.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
>
>
> Any word on Texas' regional histology meeting? I'm looking for dates and
> the registration info for my team--
>
> Thanks!
>
> Cheryl Kerry, HT(ASCP)
>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 06 Apr 2010 17:14:33 -0400
> From: "Richard Cartun" <Rcartun <@t> harthosp.org>
> Subject: Re: [Histonet] amyloid ? for Bob Richmond
> To: <histonet <@t> lists.utsouthwestern.edu>, "Carrie Disbrow"
> <disbrc <@t> shands.ufl.edu>
> Message-ID: <4BBB6BF8.7400.0077.1 <@t> harthosp.org>
> Content-Type: text/plain; charset=US-ASCII
>
> Yes, insulinomas can show amyloid; however, they are very rare tumors.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
>
>
>>>> "Carrie Disbrow" <disbrc <@t> shands.ufl.edu> 4/6/2010 5:05 PM >>>
>
>
> Hi,
> I was also told or read somewhere that insulinomas are good amyloid
> controls. I've never had a chance to test any blocks from these cases.
> What has your experience been? Thanks for your help.
> Carrie
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Tue, 6 Apr 2010 14:57:16 -0700 (PDT)
> From: Joseph Saby <saby_joseph_a <@t> yahoo.com>
> Subject: Re: [Histonet] Cleaning VIP Processor containers
> To: Anthony Reilly <Tony_Reilly <@t> health.qld.gov.au>, Brandi Higgins
> <brandihiggins <@t> gmail.com>, histonet <@t> lists.utsouthwestern.edu
> Message-ID: <276470.25744.qm <@t> web113805.mail.gq1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Brandi-
>
> The flush should be with warm water (you do not need hot), and should be
> for the fixative station(s) and the first alcohol. You only need 1 minute
> in each, just enough to get the solution to pump in.
>
> Joe Saby, BA HT
>
>
>
>
> ________________________________
> From: Anthony Reilly <Tony_Reilly <@t> health.qld.gov.au>
> To: Brandi Higgins <brandihiggins <@t> gmail.com>;
> histonet <@t> lists.utsouthwestern.edu
> Sent: Mon, April 5, 2010 10:36:42 PM
> Subject: Re: [Histonet] Cleaning VIP Processor containers
>
> Hi Brandi
>
> Do you do hot water flushes? If not the film will be a build up of the
> buffer salts from your formalin. To flush replace your formalin containers
> with hot water and write a program to run each container for 10-15
> minutes. This will not only clean the retorts but the fluid lines as well.
> This should be done weekly or at least monthly if time is an issue.
>
> regards
> Tony
>
>
>
>
>
> Tony Reilly B.Sc. , M.Sc.
> Chief Scientist, Anatomical Pathology
> Pathology Queensland-PA Laboratory
> _________________________________________________
> Clinical and Statewide Services Division| QueenslandHealth
>
> Level 1, Building 15,Princess Alexandra Hospital
> Ipswich Road,WOOLLOONGABBA Qld4102
> Ph: 07 3176 2412
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> Email: tony_reilly <@t> health.qld.gov.au
> Web: www.health.qld.gov.au/qhcss/
>
>
>
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> ------------------------------
>
> Message: 11
> Date: Tue, 06 Apr 2010 18:44:46 -0400
> From: John Kiernan <jkiernan <@t> uwo.ca>
> Subject: Re: [Histonet] Uranyl Nitrate
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <fbc4d4aa16a90.4bbb811e <@t> uwo.ca>
> Content-Type: text/plain; charset=iso-8859-1
>
> Yes. Please post the reference.
> -
> John Kiernan
> Anatomy, UWO
> London, Canada
> = = =
> ----- Original Message -----
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Date: Tuesday, April 6, 2010 17:03
> Subject: Re: [Histonet] Uranyl Nitrate
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, Dawn Oakes
> <doakes <@t> olympicmedical.org>
>
>> Yes, 1% aq. sol. of phosphotungstic acid.
>> Want the reference?
>> René J.
>>
>> --- On Tue, 4/6/10, Dawn Oakes <doakes <@t> olympicmedical.org> wrote:
>>
>>
>> From: Dawn Oakes <doakes <@t> olympicmedical.org>
>> Subject: [Histonet] Uranyl Nitrate
>> To: "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>Date: Tuesday, April 6,
>> 2010, 2:49 PM
>>
>>
>> Is there any substitutes for Uranyl Nitrate in the modified Steiner
>> Stain? How is it being disposed of?
>>
>>
>>
>> Dawn Oakes HT
>>
>> Olympic Medical Center
>>
>>
>> -----------------------------------------------------------------
>> --------
>> Confidentiality Notice: This e-mail message, including any
>> attachments, is for the sole use of the intended individual(s)
>> named above and may contain confidential, privileged, and/or
>> protected information. Any unauthorized review, use,
>> disclosure, copying, or distribution of its contents is
>> prohibited. If you are not the intended recipient, you have
>> received this email in error. If so, please notify the sender
>> immediately by reply email and delete/destroy the original and
>> all copies of this communication. Also know that Internet e-
>> mail is not secure. In choosing to communicate with Olympic
>> Medical Center by email you will assume these confidentiality
>> risks. Internet messages may become corrupted, incomplete, or
>> may incorrectly identify the sender.
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 12
> Date: Tue, 6 Apr 2010 21:21:50 -0400
> From: Brandi Higgins <brandihiggins <@t> gmail.com>
> Subject: [Histonet] Cleaning VIP Processor Containers - Summary
> To: histonet <@t> lists.utsouthwestern.edu, Katelin Lester
> <katelin <@t> cuttingedgehistology.com>
> Message-ID:
> <y2va1cc9adc1004061821y8a04018ve86889891e330666 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Thank you to everyone who responded. I got some good suggestions which I
> plan on trying.
>
> Just incase the responses I have received weren't sent to everyone, and
> also
> to make an easier-to-read reference for the future -
> Dilute acetic acid.
> xylene for waxy buildup
> Greased Lightning cleaner (store bought)
> 50/50 bleach mix - let sit for a while.
> warm water flushes in fixative and first alcohol
>
> The only response I received before changing the solutions was the dilulte
> acetic acid which we tried but did not find effective.
>
> Brandi Higgins
>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 7 Apr 2010 12:00:25 +0300
> From: Itai Moshe <itai.moshe <@t> mail.huji.ac.il>
> Subject: [Histonet] Aniline blue
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <i2kb79b24171004070200s2c754d3fn259ea17262397448 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> What type of collagen aniline blue is staining ?
>
> Thanks in advance
>
> Itai
>
>
> ------------------------------
>
> Message: 14
> Date: Wed, 7 Apr 2010 06:35:16 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Uranyl Nitrate
> To: John Kiernan <jkiernan <@t> uwo.ca>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <160131.64085.qm <@t> web65703.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Reference for phosphotungstic acid substituting uranyl nitrate as a
> sensitizer:
> Journal of Histotechnology, 2001; 24(2):113-116
> René J.
>
> --- On Tue, 4/6/10, John Kiernan <jkiernan <@t> uwo.ca> wrote:
>
>
> From: John Kiernan <jkiernan <@t> uwo.ca>
> Subject: Re: [Histonet] Uranyl Nitrate
> To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Date: Tuesday, April 6, 2010, 6:44 PM
>
>
>
> Yes. Please post the reference.
> -
> John Kiernan
> Anatomy, UWO
> London, Canada
> = = =
> ----- Original Message -----
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Date: Tuesday, April 6, 2010 17:03
> Subject: Re: [Histonet] Uranyl Nitrate
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, Dawn Oakes
> <doakes <@t> olympicmedical.org>
>
>> Yes, 1% aq. sol. of phosphotungstic acid.
>> Want the reference?
>> René J.
>>
>> --- On Tue, 4/6/10, Dawn Oakes <doakes <@t> olympicmedical.org> wrote:
>>
>>
>> From: Dawn Oakes <doakes <@t> olympicmedical.org>
>> Subject: [Histonet] Uranyl Nitrate
>> To: "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>Date: Tuesday, April 6,
>> 2010, 2:49 PM
>>
>>
>> Is there any substitutes for Uranyl Nitrate in the modified Steiner
>> Stain? How is it being disposed of?
>>
>>
>>
>> Dawn Oakes HT
>>
>> Olympic Medical Center
>>
>>
>> -----------------------------------------------------------------
>> --------
>> Confidentiality Notice: This e-mail message, including any
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>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Wed, 7 Apr 2010 16:45:46 +0300
> From: Itai Moshe <itai.moshe <@t> mail.huji.ac.il>
> Subject: [Histonet] picro sirius red, sirius red connective tissue
> stain[Scanned]
> To: histonet <@t> lists.utsouthwestern.edu, baza0013 <@t> d.umn.edu,
> David.Rushworth <@t> mail.bhrv.nwest.nhs.uk
> Message-ID:
> <s2ob79b24171004070645i516ed3a6y7f297eaad7c8e3af <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> picro Sirius red and Fast green protocol:
>
> Sirius red (Sirius Red F3B)- 0.1% in Picric acid.
> Fast Green - 0.03% in Picric acid (100%).
> Can be re-used, filter twice before each use.
>
>
> 1) Deparaffinization and Rehydration:
> A) Xylen - 10 min X2
> B) Ethanol 100% - 2 Min X2
> C) Ethanol 96% - 2 Min X1
> D) Ethanol 80% - 2 Min X1
> E) Ethanol 70% - 2 Min X1
>
> 2) Rinse in double distilled water (DDW) - 5 Min
>
> 3) Rinse in filtered Fast green - 20 Min
>
> 4) Wash with DDW ( just fill the slide box with DDW and empty).
>
> 5) Rinse in filtered Sirius red - 5 Min.
>
> 6) Wash with DDW ( just fill the slides box with DDW and empty).
>
> 7) Check in microscope, if the colors are not strong enough, repeat from
> step 2 to 7.
>
> That's it.
>
> --
> Itai Moshe
> Mark Pines lab
> Institute of Animal Sciences,Volcani Center.
> Dept. of Animal Sciences, School of Veterinary Medicine, The Hebrew
> University of Jerusalem
> Israel
>
> ------Original Message-----
> *Adam Bazama* baza0013 <@t> d.umn.edu
> <histonet%40lists.utsouthwestern.edu?Subject=%5BHistonet%5D%20sirius%20red%20connective%20tissue%20stain%5BScanned%5D&In-Reply-To=>
> *Mon Oct 6 10:27:37 CDT 2008*
>
>
> - Previous message: [Histonet] gastrin
> antibodies.....<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/039885.html>
> - Next message: [Histonet] out dated
> reagents<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/039887.html>
> - *Messages sorted by:* [ date
> ]<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/date.html#39886>
> [
> thread ]<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/thread.html#39886>
> [
> subject ]<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/subject.html#39886>
> [
> author ]<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2008-October/author.html#39886>
>
> ------------------------------
>
> Hi Dave,
>
>
>
> I noticed that you posted a request in march of 2004 on histonet for a
> Sirius red fast green staining protocol. If you do have a protocol, would
> you mind sending it to me. I have had a hard time finding one to compare
> to
> mine and my lack luster results.
>
>
>
> Thank you,
>
>
>
>
>
> Adam Bazama, B.S.
>
> Junior Scientist
>
> Lillehei Heart Institute Histology and Microscopy Core Facility
>
> University of Minnesota Medical School, Division of Cardiology
> 4-266 Nils Hasselmo Hall
> 312 Church Street SE
> Minneapolis, MN 55455
> Lab Desk: 612-625-6779
> Cell: 952-334-0607
>
> --------------------------------------------
>
> *David Rushworth* David.Rushworth <@t> mail.bhrv.nwest.nhs.uk
> <histonet%40lists.utsouthwestern.edu?Subject=%5BHistonet%5D%20sirius%20red%20connective%20tissue%20stain%5BScanned%5D&In-Reply-To=>
> *Thu Mar 4 09:00:18 CST 2004*
>
>
> - Previous message: [Histonet] reticulin procedure
> <http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/004217.html>
> - Next message: [Histonet] Research labs - charges and a question
> about outside contracting
> <http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/004219.html>
> - *Messages sorted by:* [ date ]
> <http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/date.html#4218>
> [ thread ]
> <http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/thread.html#4218>
> [ subject ]
> <http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/subject.html#4218>
> [ author ]
> <http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/author.html#4218>
>
> ------------------------------
>
> Can someone please supply method for Sirius red-fast green stain for
> connective tissue.
> Thanks in anticipation.
> Dave.
>
>
>
> ------------------------------
>
>
> - Previous message: [Histonet] reticulin
> procedure<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/004217.html>
> - Next message: [Histonet] Research labs - charges and a question about
> outside
> contracting<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/004219.html>
> - *Messages sorted by:* [ date
> ]<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/date.html#4218>
> [
> thread ]<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/thread.html#4218>
> [
> subject ]<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/subject.html#4218>
> [
> author ]<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2004-March/author.html#4218>
>
>
> ------------------------------
>
> Message: 16
> Date: Wed, 7 Apr 2010 09:46:20 -0500
> From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>
> Subject: [Histonet] frozen lung sections
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <C0B5840F14DB5B4ABF55D82A623D1E1B2BC6C5 <@t> thimailexch.THI2.COM>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi all,
>
> I have received some lung samples on OCT. These lungs have not been in
> PBS or any fixative. They went directly from dissection into OCT and
> frozen.
>
> I cannot get a section. The temp of the cryostat is -20. I have done
> some reading and it seems the best results come from immersion in PBS,
> then 4% paraformaldehyde then into sucrose. Can anyone clarify the
> procedure or offer advice? I am unfamiliar with this technique since
> most of my frozen sectioning is heart tissue and it does not present the
> same problem as the lung.
>
> Thanks,
>
> Betsy
>
>
>
> Betsy Molinari HT(ASCP)
>
> Texas Heart Institute
>
> Cardiovascular Pathology
>
> 6770 Bertner Ave
>
> MC1-283
>
> Houston,TX 77030-2607
>
> 832-355-6524
>
> 832-355-6812
>
>
>
>
>
> ------------------------------
>
> Message: 17
> Date: Wed, 7 Apr 2010 10:58:08 -0400
> From: "Michael J. Lyon, Ph.D." <lyonm <@t> upstate.edu>
> Subject: [Histonet] Old Manuals
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <00d001cad662$bc6c6580$35453080$@edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Found a couple of old manuals and will send them if you want them:
>
>
>
> Directions for use of 7203 Thomas-Franz Microtome Knife Sharpener
>
> Reference Manual No. 80-A Horizontal Sliding Microtome, Lipshaw
>
>
>
> Michael J. Lyon, Ph.D.
>
> Otolaryngology Research Lab
>
> SUNY Upstate Medical University
>
> 750 East Adams Street
>
> Syracuse, NY 13210
>
>
>
> Voice 315-464-7253
>
> Fax 315-464-5572
>
>
>
> ------------------------------
>
> Message: 18
> Date: Wed, 7 Apr 2010 09:33:07 -0600
> From: "Dishop, Megan" <Dishop.Megan <@t> tchden.org>
> Subject: [Histonet] RE: frozen lung sections
> To: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <C4052E7223019C4F9C6035E80C6F990ECCDCD180 <@t> TCHEXMBCCR2.thechildrenshospital.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> We inflate our lung tissue with a thin OCT compound (50% OCT/ 50% 0.5M
> sucrose mixture) using a needle and syringe and then embed the lung in
> 100% OCT and snap freeze. It helps for interpretation to have open
> alveoli and also gives the tissue some stability internally for frozen
> sections.
>
> Megan K. Dishop MD
> Department of Pathology, B120
> The Children's Hospital
> 13123 E. 16th Avenue
> Aurora, CO 80045
> Ph: 720-777-4337
> Fax: 720-777-7119
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Molinari,
> Betsy
> Sent: Wednesday, April 07, 2010 8:46 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] frozen lung sections
>
> Hi all,
>
> I have received some lung samples on OCT. These lungs have not been in
> PBS or any fixative. They went directly from dissection into OCT and
> frozen.
>
> I cannot get a section. The temp of the cryostat is -20. I have done
> some reading and it seems the best results come from immersion in PBS,
> then 4% paraformaldehyde then into sucrose. Can anyone clarify the
> procedure or offer advice? I am unfamiliar with this technique since
> most of my frozen sectioning is heart tissue and it does not present the
> same problem as the lung.
>
> Thanks,
>
> Betsy
>
>
>
> Betsy Molinari HT(ASCP)
>
> Texas Heart Institute
>
> Cardiovascular Pathology
>
> 6770 Bertner Ave
>
> MC1-283
>
> Houston,TX 77030-2607
>
> 832-355-6524
>
> 832-355-6812
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> --------------------------------------------------------------------------------
> CONFIDENTIALITY NOTICE: This e-mail is confidential, may be legally
> privileged,
> and for the intended recipient only. Access, disclosure, copying,
> forwarding and
> distribution by any means is strictly prohibited. If received in error,
> do not read but delete and e-mail confirmation to the sender.
> ================================================================================
>
>
>
>
> ------------------------------
>
> Message: 19
> Date: Wed, 7 Apr 2010 12:10:23 -0400
> From: Emily Sours <talulahgosh <@t> gmail.com>
> Subject: [Histonet] herring sperm dna
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <u2sb39794b1004070910p2bf0aa7bn3087f49109988a15 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Hello all!
>
> Roche has decided to stop manufacturing herring sperm DNA. I thought this
> would be an easy product to replace but apparently there's roughly ten
> bjillion versions of herring sperm DNA. I have no idea whether ours was
> sheared, sonicated, molecular biology grade, etc. It just said herring
> sperm DNA on the label. Any suggestions on a replacement? We are using
> this, btw, in hybridization buffer for RNA in situ on chick embyro
> sections
> on slides.
> I called Roche and asked tech services what their replacement would be,
> but
> they just said, here's another DNA product which uses the term
> hybridization
> in its protocol, COT Human DNA, read the tech sheet to see if that's a
> good
> replacement. Of course, the tech sheet says nothing about whether it
> would
> be good in in situ hybridization buffer, just that it's good for
> suppressing
> cross hybridization in DNA microarrays. The really frustrating thing is
> their DIG in situ hybridization protocol calls for herring sperm DNA.
>
> Emily
>
> Shall we always be content with the ancient tinned salad of the subsidized
> novel? Or the tired ice-cream of poems which cry themselves to sleep in
> the
> refrigerators of the mind?
> -Lawrence Durrell, Clea
>
>
> ------------------------------
>
> Message: 20
> Date: Wed, 7 Apr 2010 09:27:47 -0700
> From: "Kathy M. Gorham" <KMB01 <@t> grh.org>
> Subject: RE: [Histonet] Time allotted for Frozen sections
> To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <DB887A3C23A2D742912BD6AAAF17E0DA0114AB5B <@t> is-mail.grh.lan>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Thanks, to all you responded to my question about times for FS. CAP says
> 20 min. and that's what most everyone else said. Kathy
>
> ________________________________
>
> From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Tuesday, April 06, 2010 10:09 AM
> To: histonet <@t> lists.utsouthwestern.edu; Kathy M. Gorham
> Subject: Re: [Histonet] Time allotted for Frozen sections
>
>
> CAP requires an explanation as to why any FS diagnosis takes more than 20
> minutes after the sample reaches the lab.
> The average time is 15 minutes.
> René J.
>
> --- On Tue, 4/6/10, Kathy M. Gorham <KMB01 <@t> grh.org> wrote:
>
>
>
> From: Kathy M. Gorham <KMB01 <@t> grh.org>
> Subject: [Histonet] Time allotted for Frozen sections
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, April 6, 2010, 12:33 PM
>
>
> Good morning histoland. What is the maximum time allotted for frozen
> section per block? Is this written in CAP regs? JCAHO regs?
> Thanks, Kathy Gorham H.T.
>
>
> GRH National Recognition
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> Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
> Leader in Innovative Excellence 2009 awarded by the OAHHS
>
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> We will ensure ACCESS to superior QUALITY, affordable health care for all
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 21
> Date: Wed, 7 Apr 2010 12:45:03 -0400
> From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
> Subject: [Histonet] abtibodies
> To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000001cad671$abb3db60$031b9220$@com>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Histonetters
>
> I am currently looking to add three new antibodies, napsin, D2-40 and
> villin. I stain only human FFPE tissue. If anyone has used these
> antibodies
> I would appreciate your input. Thanks in advance.
>
> Regards
>
>
>
> Cindy Pyse, CLT, HT (ASCP)
>
> Histology Supervisor
>
> X-Cell Laboratories
>
> e-mail cpyse <@t> x-celllab.com
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 22
> Date: Wed, 7 Apr 2010 12:52:07 -0400
> From: "Margiotta, Michele" <MMargiotta <@t> bmhmc.org>
> Subject: [Histonet] instrument disinfection
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID:
> <D50747366FBE7F498009496ACA2D9BB00D4362 <@t> bmh-exchange.bmhmc.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi All,
>
> I need to order disinfecting solution for our grossing instruments. I
> would like to know what product most labs are using?
> Thanks for the info.
>
>
> Michele Margiotta
> BMHMC
> Histology Supervisor
> 631-654-7192
>
>
>
> DISCLAIMER:
> This e-mail and any files transmitted with it are confidential and are
> intended solely for the use of the individual or entity to which they are
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> notify the sender via return e-mail or call Brookhaven Memorial Hospital
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>
>
>
> ------------------------------
>
> Message: 23
> Date: Wed, 7 Apr 2010 12:56:55 -0400
> From: "Santiago, Albert" <Albert.Santiago <@t> uphs.upenn.edu>
> Subject: [Histonet] (no subject)
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <BB0F4D2041EED9458AD95A538DBC4CAE07080C4C <@t> uphsmbx6.UPHS.UPENN.EDU>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello fellow histonetters, we're in the process of researching bar
> coding/specimen tracking system for our lab and I was wondering if
> anyone had any recommendation of any particular system and/or vendor
> that I should look into. Thank you
>
> Albert Santiago, HT(ASCP)
> Laboratory Manager
> Dermatopathology
> 215-662-6759/6539-office
> 215-662-6150-fax
>
>
>
> The information contained in this e-mail message is intended only for the
> personal and confidential use of the recipient(s) named above. If the
> reader of this message is not the intended recipient or an agent
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> ------------------------------
>
> _______________________________________________
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>
> End of Histonet Digest, Vol 77, Issue 8
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