[Histonet] Refixing with Bouin's for Masson's Trichrome

[Rene J Buesa]

No, you should not.
What you call "refixing" is just a mordant step before Masson's trichrome and you can do that ito every section you need to stain.
 
As a matter of fact the whole tissue sample will not be "refixed" with Bouin's, and you could lose tissue orientation and other conditions now present in your sections.
René J.

--- On Wed, 4/7/10, cscampbe <@t> uci.edu <cscampbe <@t> uci.edu> wrote:


From: cscampbe <@t> uci.edu <cscampbe <@t> uci.edu>
Subject: [Histonet] Refixing with Bouin's for Masson's Trichrome
To: "HistoNet" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, April 7, 2010, 1:48 PM


Hi Histonet,

I am currently refixing my slides in Bouin's solution. Should I be
refixing the entire tissue sample? What I mean is, should I deparrafinize
my heart samples, refix in Bouin's, and reinfiltrate them before slicing
with the microtome? Or is it more effective to just refix the slides with
the heart sections on them?

Also, my procedure for the Trichrome stain has me put the slides into
phosphomolybdic acid for 5 minutes. I find that the slides come out of the
procedure very dark and purple, instead of vibrant red as expected. Is the
acid wash the problem? Should I reduce the time spent in the acid?

Thanks!
-Colin


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