[Histonet] Histotech Position in Bay Area is Still Open.

Erik Dokken Erik.Dokken <@t> onassignment.com
Mon Apr 5 13:14:15 CDT 2010


On Assignment Healthcare is currently looking for a Histo Tech for a contract assignment here in the Bay Area.

This assignment would start ASAP and will be for an indefinite period of time as it is to covering for a leave of absence.

Hours are: Monday through Friday, 5:30am to 2:00pm.

For additional information please contact me via email.

Erik Dokken
Market Leader - Northwest Region
www.oahealthcare.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, April 05, 2010 10:05 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 77, Issue 5

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Today's Topics:

   1. Re: Ab Validation (Dana Settembre)
   2. Histobath (Matthew Mincer)
   3. Happy Easter! (mohamed abd el razik)
   4. Re: Histobath (Malika Benatti)
   5. Histobath part 2 (Matthew Mincer)
   6. Re: Histobath part 2 (Lynette Pavelich)
   7. Re: Histobath (Malika Benatti)
   8. RE: 72644.18148.qm <@t> web111105.mail.gq1.yahoo.com (Mahoney,Janice A)
   9. RE: RCC (Bernice Frederick)
  10. RE: CD5 (McMahon, Loralee A)
  11. Choosing Carousel Style Tissue Processors (Parsons, Catherine)
  12. RE: CD5 (Dawson, Glen)


----------------------------------------------------------------------

Message: 1
Date: Mon, 05 Apr 2010 06:30:51 -0400
From: "Dana Settembre" <settembr <@t> umdnj.edu>
Subject: Re: [Histonet] Ab Validation
To: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <sbb983a4.054 <@t> smtpnpc.umdnj.edu>
Content-Type: text/plain; charset=US-ASCII

We have started saving these since the "NEW" question came up in June of
2009.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> Laurie Colbert <laurie.colbert <@t> huntingtonhospital.com> 04/02/10
11:19 AM >>>
When you validate a new antibody or a new antibody lot, do you save
the
slides or just the paperwork (validation report from pathologist) for
future inspection purposes?



Laurie Colbert

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------------------------------

Message: 2
Date: Mon, 05 Apr 2010 07:37:56 -0500
From: Matthew Mincer <matt <@t> techoneweb.com>
Subject: [Histonet] Histobath
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4BB9D9A4.6020203 <@t> techoneweb.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Does anyone know the "normal" operating temperature of the Thermo
Histobath? A customer has asked me to repair theirs and I want to make
sure I get the temp correct.

Thanks
Matt

--
Matthew Mincer
Tech One Biomedical Service
159 N Marion Street
PMB163
Oak Park, IL 60301
office  (708) 383-6040 X 10
cell    (708) 822-3738




------------------------------

Message: 3
Date: Mon, 5 Apr 2010 05:46:25 -0700 (PDT)
From: mohamed abd el razik <k84as <@t> yahoo.com>
Subject: [Histonet] Happy Easter!
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <794795.2296.qm <@t> web112616.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Dear histonetters
I wish to you, Happy Holidays with a lot of peace and health!

dr.Mohamed Abd el razik
Histology Dep.
Faculty of Vet. Med.
Cairo University- Egypt




------------------------------

Message: 4
Date: Mon, 5 Apr 2010 13:47:08 +0100
From: Malika Benatti <malbenatti <@t> googlemail.com>
Subject: Re: [Histonet] Histobath
To: Matthew Mincer <matt <@t> techoneweb.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <i2yafdd78481004050547jc7ff6082nface8b2e94908aa6 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi Matt,

Depending on the wax melting point which should be around 57 degree Celsius,
your water bath should be set at 50 degree Celsius.

Hope this help.

Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Children Hospital
London, WC1N 3JH
UK

Tel: (+44) 0207 4059200 Ext 5475
benatm <@t> gosh.nhs.uk




On Mon, Apr 5, 2010 at 1:37 PM, Matthew Mincer <matt <@t> techoneweb.com> wrote:

> Does anyone know the "normal" operating temperature of the Thermo
> Histobath? A customer has asked me to repair theirs and I want to make sure
> I get the temp correct.
>
> Thanks
> Matt
>
> --
> Matthew Mincer
> Tech One Biomedical Service
> 159 N Marion Street
> PMB163
> Oak Park, IL 60301
> office  (708) 383-6040 X 10
> cell    (708) 822-3738
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



--
" Smile .... it confuses people  "


------------------------------

Message: 5
Date: Mon, 05 Apr 2010 07:56:19 -0500
From: Matthew Mincer <matt <@t> techoneweb.com>
Subject: [Histonet] Histobath part 2
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4BB9DDF3.3080903 <@t> techoneweb.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

I forgot to mention, this Histobath I am talking about is the one with a
refrigerated tank. You fill the tank with a liquid (I forget what the
chemical is) and use it to quick-freeze tissue to be cut in a cryostat.

Peace
Matt

--
Matthew Mincer
Tech One Biomedical Service
159 N Marion Street
PMB163
Oak Park, IL 60301
office  (708) 383-6040 X 10
cell    (708) 822-3738




------------------------------

Message: 6
Date: Mon, 05 Apr 2010 09:03:04 -0400
From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>
Subject: Re: [Histonet] Histobath part 2
To: <histonet <@t> lists.utsouthwestern.edu>, "Matthew Mincer"
        <matt <@t> techoneweb.com>
Message-ID: <4BB9A748.59CD.00EE.0 <@t> hurleymc.com>
Content-Type: text/plain; charset=US-ASCII

Matt,
I have a copy of the instructional/operational manual from my histobath
that we purchased in 1994 from Shandon-Lipshaw.  The specifications for
its lowest temperature is -60C.  The liquid that is used is
2-methylbutane (isopentane).

I can fax you a copy if it would help.

Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
MSH Competency Coordinator
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1 <@t> hurleymc.com
ph:  810-257-9948
Lab:  810-257-9138
fax:  810-762-7082


>>> Matthew Mincer <matt <@t> techoneweb.com> 4/5/2010 8:56 AM >>>
I forgot to mention, this Histobath I am talking about is the one with
a
refrigerated tank. You fill the tank with a liquid (I forget what the
chemical is) and use it to quick-freeze tissue to be cut in a
cryostat.

Peace
Matt

--
Matthew Mincer
Tech One Biomedical Service
159 N Marion Street
PMB163
Oak Park, IL 60301
office  (708) 383-6040 X 10
cell    (708) 822-3738


_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 7
Date: Mon, 5 Apr 2010 13:59:06 +0100
From: Malika Benatti <malbenatti <@t> googlemail.com>
Subject: Re: [Histonet] Histobath
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <x2lafdd78481004050559lf76cee5fk1677e4f09a6adf3c <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Sorry for that Matt,

In this case the internal temperature of the cryostat chamber should be set
at -20 to -22 degree Celsius, not sure what would the actual temperature of
the histobath itself but our thermo scientific cryostat has the following
settings:

   - the cryobar is set at -18 Degree Celsius,
   - the Cryo chamber at - 22 degree Celsius.


Hope this help

Malika Benatti

Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Children Hospital
London, WC1N 3JH
UK

Tel: (+44) 0207 4059200 Ext 5475
benatm <@t> gosh.nhs.uk
- Show quoted text -

On Mon, Apr 5, 2010 at 1:51 PM, Matthew Mincer <matt <@t> techoneweb.com> wrote:

> Thanks, but the Histobath I was talking about is a freezing device for
> cryostats.
>
> Matt
>
> Malika Benatti wrote:
>
>> Hi Matt,
>>
>> Depending on the wax melting point which should be around 57 degree
>> Celsius, your water bath should be set at 50 degree Celsius.
>>
>> Hope this help.
>>
>> Malika Benatti BSc MIBMS
>> Specialist Biomedical Scientist
>> Great Ormond Street Children Hospital
>> London, WC1N 3JH
>> UK
>>
>> Tel: (+44) 0207 4059200 Ext 5475
>> benatm <@t> gosh.nhs.uk <mailto:benatm <@t> gosh.nhs.uk>
>>
>>
>>
>>
>>
>> On Mon, Apr 5, 2010 at 1:37 PM, Matthew Mincer <matt <@t> techoneweb.com<mailto:
>> matt <@t> techoneweb.com>> wrote:
>>
>>    Does anyone know the "normal" operating temperature of the Thermo
>>    Histobath? A customer has asked me to repair theirs and I want to
>>    make sure I get the temp correct.
>>
>>    Thanks
>>    Matt
>>
>>    --     Matthew Mincer
>>    Tech One Biomedical Service
>>    159 N Marion Street
>>    PMB163
>>    Oak Park, IL 60301
>>    office  (708) 383-6040 X 10
>>    cell    (708) 822-3738
>>
>>
>>    _______________________________________________
>>    Histonet mailing list
>>    Histonet <@t> lists.utsouthwestern.edu
>>    <mailto:Histonet <@t> lists.utsouthwestern.edu>
>>
>>    http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>>
>> --
>> " Smile .... it confuses people  "
>>
>
>
> --
> Matthew Mincer
> Tech One Biomedical Service
> 159 N Marion Street
> PMB163
> Oak Park, IL 60301
> office  (708) 383-6040 X 10
> cell    (708) 822-3738
>
>


--
" Smile .... it confuses people  "


------------------------------

Message: 8
Date: Mon, 5 Apr 2010 08:08:17 -0500
From: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>
Subject: RE: [Histonet] 72644.18148.qm <@t> web111105.mail.gq1.yahoo.com
To: 'Joe Nocito' <jnocito <@t> satx.rr.com>, Andrew Burgeson
        <napoli <@t> siscom.net>,    "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A050 <@t> EXCHMBC2.ad.ah.local>
Content-Type: text/plain; charset="us-ascii"

Talk about politically incorrect.  "Pregnant, out of wedlock 16 year olds?"  Are you kidding me?  Seriously AB?
Jan


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Thursday, April 01, 2010 7:42 PM
To: Andrew Burgeson; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] 72644.18148.qm <@t> web111105.mail.gq1.yahoo.com

I've seen lab get accredited one month, only to have CLIA come through the
next month and rip that lab apart. I have been through CAP inspections that
consisted of the inspector looking only at slides while he checked off the
checklist to inspectors carrying their own thermometers and testing the temp
of the paraffin baths on the tissue processors. This is supposed to be a
peer review, often it's a witch hunt. I had one CAP team leader tell me that
my lab was the last lab he was going to inspect because he dropped out of
CAP and went with JCAHO, which was approved by his hospital administrator.
Does anyone know why CAP split the "processing" and "grossing parts anyway?
I thought it was a stupid idea in the first place. But then again, I've
never been known to be politically correct

Joe
----- Original Message -----
From: "Andrew Burgeson" <napoli <@t> siscom.net>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, April 01, 2010 12:26 PM
Subject: [Histonet] 72644.18148.qm <@t> web111105.mail.gq1.yahoo.com


> Sheesh is right, J.
>
> CAP is all politics as far as I am concerned. It is all
> about protecting the careers and paychecks of the general
> pathology community.
>
> I am thouroughly unimpressed with JCAHO, CAP et al.
>
> If all you need to legally run a laboratory is to be CLIA
> inspected, then WHY BOTHER with these subjective entities?
>
>
> The BS I have heard over the last few months concerning MOHS
> surgery specimens is one glaring example of the limitations
> CAP has in understanding fully certain nuances of the lab
> trade.
>
> Ridiculous. Unless you want the marketing and potential
> "perception" that you are better covered from a legal
> standpoint, CAP certs are worthless.
>
> The more I hear about CAP certifications, the more I see it
> as a certain community of individuals who are protecting
> their perceived "TURF."
>
> In the end, the pathologists in the group and in the
> facility in which you are working have to take
> responsibility for these matters. If the docs think a CAP
> cert is necessary, then do it and live with it. If not, then
> consider yourself lucky to not have to see these people in
> your lab.
>
> I have been through MANY CAP inspections in and out of the
> military. For the most part, though, I see people paying
> this organization to inspect their lab as the same thing as
> "burning a pinch of incense in honor of great Caesar, ruler
> of Rome." It will get you some kudos, but tangibly not
> change much at all if your pathologists or HR $ hiring hands
> want to pocket more $ as a result of hiring "pregnant out of
> wedlock 16 year olds" to gross tissue and cut slides.
>
> Seen it.
>
> AB
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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------------------------------

Message: 9
Date: Mon, 5 Apr 2010 09:00:58 -0500
From: "Bernice Frederick" <b-frederick <@t> northwestern.edu>
Subject: RE: [Histonet] RCC
To: "'Howery, Jeffrey'" <JHowery2 <@t> yrmc.org>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <6D9B42048B2E43C4AEEC2B630377C443 <@t> lurie.northwestern.edu>
Content-Type: text/plain;       charset="US-ASCII"

RCC from Novacastra (Leica)


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Howery,
Jeffrey
Sent: Friday, April 02, 2010 12:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RCC

What are people using for a Renal cell Carcinoma. ( this is a test )

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------------------------------

Message: 10
Date: Mon, 5 Apr 2010 10:06:17 -0400
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: [Histonet] RE: CD5
To: "Howery, Jeffrey" <JHowery2 <@t> yrmc.org>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <C27AA2A01CEF31469813089E226F582E838C06C6 <@t> urmcms7.urmc-sh.rochester.edu>

Content-Type: text/plain; charset="us-ascii"

High pH Retrieval for 20 minutes in the PT module

20 minute incubation of CD5
15 minute mouse linker
20 Minute incubation Flex HRP
10 minute DAB
5 minute Hematoxylin


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [JHowery2 <@t> yrmc.org]
Sent: Friday, April 02, 2010 4:57 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD5

Can anyone share thier protocol for CD5. We use the Dako platform. Thanks

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------------------------------

Message: 11
Date: Mon, 5 Apr 2010 10:26:08 -0400
From: "Parsons, Catherine" <cparsons <@t> vt.edu>
Subject: [Histonet] Choosing Carousel Style Tissue Processors
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <F432A8A667BE1A43BC6B09C8611856B425A4C25AAF <@t> fangorn.cc.w2k.vt.edu>
Content-Type: text/plain; charset="us-ascii"


I am in the process of selecting a new tissue processor for my lab, and have narrowed the search to two models.   Both are carousel style tissue processors.  We are a small, low volume research lab, so the carousel style fits well with our processing and space needs.  I would appreciate any information/opinions  that anyone has about these two models and manufacturers.  Major lab equipment purchases seem to be on a 20 year cycle here, so I need to choose carefully and well!

My choices are the Leica TP 1020 and the Thermo Scientific Microm STP 120 Spin tissue processor.

Has anyone found the spin function on the Microm unit to be useful, or just another part to break down and need replacement?

Thanks in advance for any information that you can offer.


Cathy Parsons
cparsons <@t> vt.edu




------------------------------

Message: 12
Date: Mon, 5 Apr 2010 09:55:30 -0500
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: [Histonet] RE: CD5
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <B3D65550856D0146B900D401EE313D4B034042CC <@t> dynams.dynacaremilwaukee.com>

Content-Type: text/plain;       charset="iso-8859-1"

Jeff,

Thermo Scientific (Formally NeoMarkers) CD5 (Clone SP19):  Catalogue# RM-9119-S.

Concentration of 1:75 diluted w/Dako diluent.

Antigen Retrieval: PH6.0 Citrate Target Retrieval (DAKO) for 35 min. @ 99 degrees C, followed by a 20 min. room temp. cool down.

35 minute primary antibody application.

30 minute Envision+ polyclonal (DAKO) secondary antibody detection.

7 minute DAB+ (DAKO).

Counter-stain as you like.

Results on this antibody are excellent.


Best of Luck,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [JHowery2 <@t> yrmc.org]
Sent: Friday, April 02, 2010 4:57 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD5

Can anyone share thier protocol for CD5. We use the Dako platform. Thanks

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