[Histonet] RE: CD5

[McMahon, Loralee A]

High pH Retrieval for 20 minutes in the PT module

20 minute incubation of CD5
15 minute mouse linker
20 Minute incubation Flex HRP
10 minute DAB
5 minute Hematoxylin


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [JHowery2 <@t> yrmc.org]
Sent: Friday, April 02, 2010 4:57 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD5

Can anyone share thier protocol for CD5. We use the Dako platform. Thanks

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