[Histonet] Histobath

[Malika Benatti]

Sorry for that Matt,

In this case the internal temperature of the cryostat chamber should be set
at -20 to -22 degree Celsius, not sure what would the actual temperature of
the histobath itself but our thermo scientific cryostat has the following
settings:

   - the cryobar is set at -18 Degree Celsius,
   - the Cryo chamber at - 22 degree Celsius.


Hope this help

Malika Benatti

Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Children Hospital
London, WC1N 3JH
UK

Tel: (+44) 0207 4059200 Ext 5475
benatm <@t> gosh.nhs.uk
- Show quoted text -

On Mon, Apr 5, 2010 at 1:51 PM, Matthew Mincer <matt <@t> techoneweb.com> wrote:

> Thanks, but the Histobath I was talking about is a freezing device for
> cryostats.
>
> Matt
>
> Malika Benatti wrote:
>
>> Hi Matt,
>>
>> Depending on the wax melting point which should be around 57 degree
>> Celsius, your water bath should be set at 50 degree Celsius.
>>
>> Hope this help.
>>
>> Malika Benatti BSc MIBMS
>> Specialist Biomedical Scientist
>> Great Ormond Street Children Hospital
>> London, WC1N 3JH
>> UK
>>
>> Tel: (+44) 0207 4059200 Ext 5475
>> benatm <@t> gosh.nhs.uk <mailto:benatm <@t> gosh.nhs.uk>
>>
>>
>>
>>
>>
>> On Mon, Apr 5, 2010 at 1:37 PM, Matthew Mincer <matt <@t> techoneweb.com<mailto:
>> matt <@t> techoneweb.com>> wrote:
>>
>>    Does anyone know the "normal" operating temperature of the Thermo
>>    Histobath? A customer has asked me to repair theirs and I want to
>>    make sure I get the temp correct.
>>
>>    Thanks
>>    Matt
>>
>>    --     Matthew Mincer
>>    Tech One Biomedical Service
>>    159 N Marion Street
>>    PMB163
>>    Oak Park, IL 60301
>>    office  (708) 383-6040 X 10
>>    cell    (708) 822-3738
>>
>>
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>>
>>
>>
>> --
>> " Smile .... it confuses people  "
>>
>
>
> --
> Matthew Mincer
> Tech One Biomedical Service
> 159 N Marion Street
> PMB163
> Oak Park, IL 60301
> office  (708) 383-6040 X 10
> cell    (708) 822-3738
>
>


-- 
" Smile .... it confuses people  "