[Histonet] mounting media

[John Kiernan]

I've used both the cytoseal mountants (with numbers that indicate viscosity) for several years. These poly(methylmethacrylate) media come in very convenient plastic squeezy bottles for application. They are less expensive than entellan, the classic polymethacrylate mountant, which has to be dispensed from its bottle with a glass rod. 

With cytoseal mounting media, lines of little bubbles frequently form, very near the edges of the coverslip. I don't know why. Bubbles at the edge don't matter if the sections are small and centrally located on the slide. I've not seen these lines of bubbles with entellan or with DPX, a non-fluorescent polystyrene medium.

John Kiernan
Anatomy,  UWO
London,  Canada
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
tigger13b <@t> aol.com
Sent: Wednesday, March 31, 2010 1:51 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] MM24 mounting media

Hello,

Does anyone use MM24 mounting media from surgipath?  We use cytoseal 60
but I can get the MM24 at a lower price.  If anyone has used this, could
you tell me whether you were satisfied with the product or not?  Thanks
so much.

Brandi Higgins
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