[Histonet] CD31 Mouse Liver Staining

Fri Sep 25 08:29:00 CDT 2009

Hi Jeff,

I have had best results using Zinc Fixative for CD31 (PECAM1) staining in mouse tissues.  You can either post fix cryosections or immersion fix tissues trimmed thin (~4mm). Zinc Fixative is available from BD/Pharmingen or you can prepare it.  Email me if you want details on preparing it yourself.

Good luck,
Jameel

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Today's Topics:

   1. Re: Orienting GI biopsies during embedding (Rene J Buesa)
   2. Re: Histobath Frozen Sections (Robert Richmond)
   3. Re: Orienting GI biopsies during embedding (Robert Richmond)
   4. Heidelberger Pinzette (Gudrun Lang)
   5. MSI testing (Richard Cartun)
   6. Steiner and Steiner (Wahlberg, Nikki)
   7. SOX10 (Martin, Erin)
   8. RE: Steiner and Steiner (Jodie Robertson)
   9. RE: MSI testing (Weems, Joyce)
  10. Need a NSH S/C roommate (Marquisha Paul)
  11. CD31 Mouse Liver Staining (JEFFREY S HARDING)
  12. FW: [Histonet] Steiner and Steiner (Wahlberg, Nikki)
  13. Guniea pig anti-Doublecortin & Goat-Guinea pig secondary
      antibody on rat tissue ( ti fei )
  14. MOLECULAR LAB QUESTION (Maria Katleba)
  15. cap survays (Gonzales, Edith)
  16. Re: Steiner and Steiner (Rene J Buesa)
  17. RE: cap survays (Morken, Tim)
  18. Re: ISH on decaled bone (Johnson, Teri)
  19. Re: License (Johnson, Teri)
  20. B Plus fixative on bone marrow cores (Della Speranza, Vinnie)
  21. RE: Orienting GI biopsies during embedding (Tony Henwood)
  22. RE: Steiner and Steiner (Tony Henwood)
  23. RE: B Plus fixative on bone marrow cores (Lee & Peggy Wenk)
  24. RE: cap survays (Tony Henwood)
  25. See you at the NSH (Tony Henwood)
  26. Rhodanine Copper stain (Carrie Disbrow)

----------------------------------------------------------------------

Message: 1
Date: Thu, 24 Sep 2009 10:11:17 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Orienting GI biopsies during embedding
To: "histonet <@t> lists.utsouthwestern.edu"
    <histonet <@t> lists.utsouthwestern.edu>,    ssylvest <@t> cinci.rr.com 
Message-ID: <500291.35986.qm <@t> web65713.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

There is a very fine Technical Note by I. Dimenstein in the last issue of the JOH that can help you solve this problem.
René J.

--- On Thu, 9/24/09, ssylvest <@t> cinci.rr.com <ssylvest <@t> cinci.rr.com> wrote:

From: ssylvest <@t> cinci.rr.com <ssylvest <@t> cinci.rr.com>
Subject: [Histonet] Orienting GI biopsies during embedding
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, September 24, 2009, 12:45 PM

Everyone,

We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it.

Sabina Sylvest
Department of Pathology
Cincinnati Children's Hospital
ssylvest <@t> cinci.rr.com 
(513)803-0741

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------------------------------

Message: 2
Date: Thu, 24 Sep 2009 13:15:16 -0400
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Histobath Frozen Sections
To: histonet <@t> lists.utsouthwestern.edu 
Message-ID:
    <abea52a60909241015i654ccf9cm4b38972efeae3689 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

I've posted a number of times about the Histobath. I don't know
anything new besides the stuff I've already posted.

Melanie S. White, MT(ASCP) asks:
>>P.S.  Are there any other Samurai Pathologists available? We need one.<<

Hey, find me a way to get a South Carolina medical license at the age
of 70 and we'll talk!

Bob Richmond
Samurai Pathologist
Knoxville TN

------------------------------

Message: 3
Date: Thu, 24 Sep 2009 13:27:10 -0400
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Orienting GI biopsies during embedding
To: histonet <@t> lists.utsouthwestern.edu 
Message-ID:
    <abea52a60909241027k389ccf14t5c74c304d7bfc961 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Sabina Sylvest at Cincinnati Children's Hospital asks:

>>We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it.<<

At the very least, I'd use an OptiVISOR magnifier with 3 diopter or 4
diopter lenses - I've mentioned this item on Histonet before. See
www.doneganoptical.com/optivisor.php - Donegan doesn't retail, but you
can get the item from Amazon.

If I had one, I'd want to use a stereo dissecting microscope with 10
and 20 power magnification, but not many pathology services have them.

Orienting GI biopsies is more important than most people realize. As
public awareness of celiac disease increases, we're going to have to
improve our handling of upper small bowel biopsy specimens. I'm
thinking of

     examining the fixed gross specimen with a dissecting microscope,
as part of the gross description
     embedding with magnification
     doing special stains routinely - PAS (for Whipple cells) and CD3
(for infiltrating T lymphocytes in the surface epithelium)

Is anyone on Histonet doing any of these things?

Bob Richmond
Samurai Pathologist
Knoxville TN

------------------------------

Message: 4
Date: Thu, 24 Sep 2009 19:28:05 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: [Histonet] Heidelberger Pinzette
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <8E65CB18784E4A8782468C23EA49B3C4 <@t> dielangs.at>
Content-Type: text/plain;    charset="us-ascii"

Does anybody know a distributor of the classic "Heidelberger Pinzette"
heated forcep?

Gudrun Lang

Histolab, AKH Linz, Austria

------------------------------

Message: 5
Date: Thu, 24 Sep 2009 13:38:25 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] MSI testing
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4ABB7650.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII

How many of you that do IHC and/or PCR testing for microsatellite instability (MSI) have your requests go through a genetic counselor?  Thanks!

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

------------------------------

Message: 6
Date: Thu, 24 Sep 2009 12:38:14 -0500
From: "Wahlberg, Nikki" <Nikki.Wahlberg <@t> bsci.com>
Subject: [Histonet] Steiner and Steiner
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <5BF2F9FB24C0DC499CD3C4BB6F7B4481013E24DD <@t> MAPMAIL02.bsci.bossci.com>
Content-Type: text/plain;    charset="us-ascii"

Does anyone have a protocol for the Steiner and Steiner stain that does
not use Uranyl Nitrate?  Our EHS department will not allow us to order
this chemical anymore because it is too expensive to dispose of.  If
anyone has any methods of disposal that are not too pricey I would be
interested in them as well.

Thank you very much,
Nikki

Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
763-694-5739
wahlbern <@t> bsci.com 

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------------------------------

Message: 7
Date: Thu, 24 Sep 2009 10:39:46 -0700
From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
Subject: [Histonet] SOX10
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <379A927A452F3D43A3C8705F4E67905F0CE8271200 <@t> EX05.net.ucsf.edu>
Content-Type: text/plain; charset="iso-8859-1"

Hi,  Would anyone using SOX10 antibody please tell me where they buy it?  If you have a procedure that you would be willing to share as well I would be very appreciative.  Thank you!

Erin Martin
UCSF Dermatopathology

------------------------------

Message: 8
Date: Thu, 24 Sep 2009 10:49:33 -0700
From: "Jodie Robertson" <jrobertson <@t> pathologysciences.com>
Subject: RE: [Histonet] Steiner and Steiner
To: "Wahlberg, Nikki" <Nikki.Wahlberg <@t> bsci.com>,
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <518CD6920AA7154193CBE5977CD880733A8BA1 <@t> psmgsrv2.PSMG.local>
Content-Type: text/plain;    charset="us-ascii"

Check with Newcomer Supply as they have a Modified Steiner Kit without
Uranyl Nitrate.

Jodie Robertson, HT(ASCP) QIHC
                    Pathology Sciences Medical Group
                        Histology Day Supervisor
                        Chico, CA  95926
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Wahlberg, Nikki
Sent: Thursday, September 24, 2009 10:38 AM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Steiner and Steiner

Does anyone have a protocol for the Steiner and Steiner stain that does
not use Uranyl Nitrate?  Our EHS department will not allow us to order
this chemical anymore because it is too expensive to dispose of.  If
anyone has any methods of disposal that are not too pricey I would be
interested in them as well.

Thank you very much,
Nikki

Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
763-694-5739
wahlbern <@t> bsci.com 

CONFIDENTIALITY NOTICE:  This e-mail transmission may contain
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distribution or copying of this transmittal or its attachments, if any,
is prohibited.  If you have received this e-mail transmission in error,
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inbox.

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 9
Date: Thu, 24 Sep 2009 13:49:41 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] MSI testing
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <B36E22177C738742AE407DBA366FFD4A0658F7 <@t> ITSSSXM02V1.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

Not us!!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Thursday, September 24, 2009 13:38
To: Histonet
Subject: [Histonet] MSI testing

How many of you that do IHC and/or PCR testing for microsatellite
instability (MSI) have your requests go through a genetic counselor?
Thanks!

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic
Pathology Hartford Hospital 80 Seymour Street Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

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------------------------------

Message: 10
Date: Thu, 24 Sep 2009 13:50:14 -0400
From: "Marquisha Paul" <MPaul <@t> Alltech.com>
Subject: [Histonet] Need a NSH S/C roommate
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <421EE4FDC90A744CB1DDDDD21A1BC56E0B2B92D1 <@t> ERGO.Alltech-bio.com>
Content-Type: text/plain;    charset="us-ascii"

I would like to share a room for the NSH Symposium/Convention in
Birmingham to help save on costs.  I have a room reserved at DoubleTree
from Friday - Wednesday, but if you already have a room reserved and are
looking for someone to share costs, I can do that too.

Send me an email at mpaul <@t> alltech.com if you are interested

Thanks a lot!

Marquisha Paul

Research Lab Tech

Alltech, Inc.

Lexington, KY

------------------------------

Message: 11
Date: Thu, 24 Sep 2009 13:46:16 -0500
From: JEFFREY S HARDING <jsharding <@t> wisc.edu>
Subject: [Histonet] CD31 Mouse Liver Staining
To: histonet <@t> lists.utsouthwestern.edu 
Message-ID: <7050ac4f65505.4abb7828 <@t> wiscmail.wisc.edu>
Content-Type: text/plain; charset=us-ascii

Hello,

    I've tried a dozen times to get good fluorescent CD31 staining (endothelial marker) on frozen mouse liver sections.   

   I'm using the standard pharmogen Anti-CD31 primary with a secondary FITC label, and get a very weak (though noticable) signal. 

  My question is regarding fixation methods IMMEDIATELY after dissection.  Does anyone have a good summary of fixation methods prior to freezing and their effects on CD31 staining?  Does the exact fixation even matter that much?  Everyone seems to have something different.   

Thanks!
Jeff

------------------------------

Message: 12
Date: Thu, 24 Sep 2009 14:01:05 -0500
From: "Wahlberg, Nikki" <Nikki.Wahlberg <@t> bsci.com>
Subject: FW: [Histonet] Steiner and Steiner
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <5BF2F9FB24C0DC499CD3C4BB6F7B448103400361 <@t> MAPMAIL02.bsci.bossci.com>
Content-Type: text/plain;    charset="us-ascii"

We already use the kit from Sigma but it is the 1% Uranyl Nitrate waste
that is the issue.  I will check with Newcommer to see if their kit will
work.

Thanks you!
Nikki

-----Original Message-----
From: Bernice Frederick [mailto:b-frederick <@t> northwestern.edu] 
Sent: Thursday, September 24, 2009 1:44 PM
To: Wahlberg, Nikki
Subject: RE: [Histonet] Steiner and Steiner

Nikki,
What if you but the Sigma kit that has the 1% solution in it already? 
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Wahlberg,
Nikki
Sent: Thursday, September 24, 2009 12:38 PM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Steiner and Steiner

Does anyone have a protocol for the Steiner and Steiner stain that does
not use Uranyl Nitrate?  Our EHS department will not allow us to order
this chemical anymore because it is too expensive to dispose of.  If
anyone has any methods of disposal that are not too pricey I would be
interested in them as well.

Thank you very much,
Nikki

Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
763-694-5739
wahlbern <@t> bsci.com 

CONFIDENTIALITY NOTICE:  This e-mail transmission may contain
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the individual or entity named in the e-mail address.  Any unauthorized
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is prohibited.  If you have received this e-mail transmission in error,
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 13
Date: Fri, 25 Sep 2009 03:33:31 +0800
From: " ti fei " <tifei <@t> foxmail.com>
Subject: [Histonet] Guniea pig anti-Doublecortin & Goat-Guinea pig
    secondary    antibody on rat tissue
To: " Histonet " <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <tencent_5C2E116D07F5C21C27B39AA8 <@t> qq.com>
Content-Type: text/plain;    charset="ISO-8859-1"

it seems when I use guniea pig-anti-doublecortin (DCX) with secondary antibody from invitrogen goat-anti guinea pig Alexa 488 or 568. I always get staining on blood vessel-like structures. 

Anyone has such experiences before? Do goat-anti-guinea pig IgG will cross react with rat IgG ?
Or it is due to primary antibody problem - which is unlikely because the antibody has been used without a problem in other labs.

------------------------------

Message: 14
Date: Thu, 24 Sep 2009 12:39:30 -0700
From: Maria Katleba <Maria.Katleba <@t> stjoe.org>
Subject: [Histonet] MOLECULAR LAB QUESTION
To: "histonet <@t> lists.utsouthwestern.edu"
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <97C02552ECB11346877D3E83CF833ABD13ED9B728E <@t> SJSNT-SCMAIL03.stjoe.org>
Content-Type: text/plain; charset="us-ascii"

Two questions (if anyone can answer):

(1) What tests are you doing if you in fact have a 'molecular lab'?

(2) Who runs the tests...Histotech or CLS ?

Thanks!

Maria Katleba HT(ASCP), MS
Pathology Dept. Mgr.
Queen of the Valley Medical Center
707-294-9229 cell
707-252-4411 x3689

________________________________
Notice from St. Joseph Health System:
Please note that the information contained in this message may be privileged and confidential and protected from disclosure.

------------------------------

Message: 15
Date: Thu, 24 Sep 2009 11:50:12 -0800
From: "Gonzales, Edith" <Edith.Gonzales <@t> providence.org>
Subject: [Histonet] cap survays
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <E66025C48FBA6C43888E7C65EACEF3A636C247 <@t> wn1286.ak.providence.org>
Content-Type: text/plain; charset="iso-8859-1"

To all,

I would like to know what everyone is doing with the cap survay now that it is scoring staining performance and not detremining a diagnosis.  Our paths want us to score then and they not be involved.  Is this what everone else is doing?

Edie

DISCLAIMER:
This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message.

This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message.

------------------------------

Message: 16
Date: Thu, 24 Sep 2009 12:59:58 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Steiner and Steiner
To: histonet <@t> lists.utsouthwestern.edu, NikkiWahlberg
    <Nikki.Wahlberg <@t> bsci.com>
Message-ID: <520616.30496.qm <@t> web65712.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Under separate cover I am sending you a procedures for modified Steiner without uranyl nitrate.
René J.

--- On Thu, 9/24/09, Wahlberg, Nikki <Nikki.Wahlberg <@t> bsci.com> wrote:

From: Wahlberg, Nikki <Nikki.Wahlberg <@t> bsci.com>
Subject: [Histonet] Steiner and Steiner
To: histonet <@t> lists.utsouthwestern.edu 
Date: Thursday, September 24, 2009, 1:38 PM

Does anyone have a protocol for the Steiner and Steiner stain that does
not use Uranyl Nitrate?  Our EHS department will not allow us to order
this chemical anymore because it is too expensive to dispose of.  If
anyone has any methods of disposal that are not too pricey I would be
interested in them as well.

Thank you very much,
Nikki

Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
763-694-5739
wahlbern <@t> bsci.com 

CONFIDENTIALITY NOTICE:  This e-mail transmission may contain
confidential or legally privileged information that is intended only for
the individual or entity named in the e-mail address.  Any unauthorized
distribution or copying of this transmittal or its attachments, if any,
is prohibited.  If you have received this e-mail transmission in error,
please reply to the sender, so that Boston Scientific Corporation can
arrange for proper delivery, then please delete the message from your
inbox.

_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 17
Date: Thu, 24 Sep 2009 13:16:54 -0700
From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] RE: cap survays
To: "Gonzales, Edith" <Edith.Gonzales <@t> providence.org>,
    "histonet <@t> lists.utsouthwestern.edu"
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <1AAF670737F193429070841C6B2ADD4CF6B66388 <@t> EXMBMCB15.ucsfmedicalcenter.org>

Content-Type: text/plain; charset=us-ascii

Technologists can certainly be trained to do the scoring properly but our pathologists score them and are fully involved in the evaluations, as well as reviewing challenge slides for submission. There are also sometimes interpretation questions that have to be done on-line that the pathologists have to do, for instance for Her2. You have to ask the question of who is doing the interpretation in "real life," the techs or the pathologist? The survey is supposed to be done the same way you do it in your lab in your daily work so take that as the starting point of the discussion.

I think it defeats the purpose if the pathologists are not involved since it is a test of the total quality system. Are they or aren't they part of the system? 

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gonzales, Edith
Sent: Thursday, September 24, 2009 12:50 PM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] cap survays

To all,

I would like to know what everyone is doing with the cap survay now that it is scoring staining performance and not detremining a diagnosis.  Our paths want us to score then and they not be involved.  Is this what everone else is doing?

Edie

DISCLAIMER:
This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message.

This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message.

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------------------------------

Message: 18
Date: Thu, 24 Sep 2009 16:40:54 -0500
From: "Johnson, Teri" <TJJ <@t> stowers.org>
Subject: [Histonet] Re: ISH on decaled bone
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <BD62CBAC4395B94096109020651BE2EC12CE721BAA <@t> exchmb-02.stowers-institute.org>

Content-Type: text/plain; charset="us-ascii"

Gudrun is absolutely correct. The HCl destroyed your nucleic acids. The best decalcifier for RNA or DNA is EDTA.

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110

------------------------------

Message: 19
Date: Thu, 24 Sep 2009 16:52:12 -0500
From: "Johnson, Teri" <TJJ <@t> stowers.org>
Subject: [Histonet] Re: License
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <BD62CBAC4395B94096109020651BE2EC12CE721BAC <@t> exchmb-02.stowers-institute.org>

Content-Type: text/plain; charset="us-ascii"

Joyce,

You might have some luck calling the following places:

Physician's Reference Lab, Overland Park, KS - Donna Miller

Shawnee Mission Medical Center, Overland Park, KS

North Kansas City Hospital - N. KC, MO - Nancy Warren

University of Kansas Medical Center, Kansas City, KS - Timothy Chilton

Midwest Anatomic Pathology Lab, Overland Park, KS

Litton Laboratories, Blue Springs, MO

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110

------------------------------

Message: 20
Date: Thu, 24 Sep 2009 18:06:45 -0400
From: "Della Speranza, Vinnie" <dellav <@t> musc.edu>
Subject: [Histonet] B Plus fixative on bone marrow cores
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <E58D1CD977E29C46A167806513B0457798D4873DFC <@t> EVS5.clinlan.local>
Content-Type: text/plain; charset="us-ascii"

I would appreciate comments from anyone using this fixative for bone marrow cores. Specifically I am interested in learning how long you are fixing your samples in B Plus.
Manufacturer recommends "at least two hours" but we are seeing artifacts that concern me that 2 hrs may be insufficient.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974

------------------------------

Message: 21
Date: Fri, 25 Sep 2009 08:40:14 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Orienting GI biopsies during embedding
To: <ssylvest <@t> cinci.rr.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC5552068537B1 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"

I would recommend you try to embed them on edge.
Even if very small, you will be surprised how many are correctly
embedded

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
ssylvest <@t> cinci.rr.com 
Sent: Friday, 25 September 2009 2:46 AM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Orienting GI biopsies during embedding

Everyone,

We are having a heck of a time orienting GI biopsies but esophageal
biopsies in particular, during embedding. If anyone could share their
secret for getting these tiny specks of semi-transparent tissue
oriented, we would greatly appreciate it.

Sabina Sylvest
Department of Pathology
Cincinnati Children's Hospital
ssylvest <@t> cinci.rr.com 
(513)803-0741

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Message: 22
Date: Fri, 25 Sep 2009 09:11:00 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Steiner and Steiner
To: "Wahlberg, Nikki" <Nikki.Wahlberg <@t> bsci.com>,
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC5552068537B2 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"

Nikki,

The sensitisation step, from my experience, improves the silver
deposition on the bacteria and decreases background staining. Margeson &
Chappman (1996 Use of Zinc Formalin as a Sensitizer in Silver Stains for
Spirochetes J Histotechnol 19(2):135-138) substituted zinc formalin for
uranyl nitrate in the Steiner techniques and found the resulting
spirochete staining to be as good as or better than when uranyl nitrate
was used.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Wahlberg, Nikki
Sent: Friday, 25 September 2009 3:38 AM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Steiner and Steiner

Does anyone have a protocol for the Steiner and Steiner stain that does
not use Uranyl Nitrate?  Our EHS department will not allow us to order
this chemical anymore because it is too expensive to dispose of.  If
anyone has any methods of disposal that are not too pricey I would be
interested in them as well.

Thank you very much,
Nikki

Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
763-694-5739
wahlbern <@t> bsci.com 

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Message: 23
Date: Thu, 24 Sep 2009 19:22:24 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: RE: [Histonet] B Plus fixative on bone marrow cores
To: "'Della Speranza, Vinnie'" <dellav <@t> musc.edu>,    "'Histonet'"
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1072842512EE4F958AA1E6638D38A93E <@t> HPPav2>
Content-Type: text/plain;    charset="us-ascii"

One of my students, a few years ago, tried several different zinc formalins
from different companies, on bone marrow biopsies. At 2 hours fixation, we
saw a lot of smudgy, pale baby blue nuclei. At 3 hours, we were seeing
nuclear detail of the usual hematoxylin blue. 4 hours was slightly better,
but our pathologists thought that the slight improvement wasn't needed to
make a diagnosis - they could do it just as well at 3 hours, and it saved an
hour. It didn't matter which zinc formalin we used, 3 hours seemed to be the
key. So we now make 3 hours as the minimum.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Della
Speranza, Vinnie
Sent: Thursday, September 24, 2009 6:07 PM
To: Histonet
Subject: [Histonet] B Plus fixative on bone marrow cores

I would appreciate comments from anyone using this fixative for bone marrow
cores. Specifically I am interested in learning how long you are fixing your
samples in B Plus.
Manufacturer recommends "at least two hours" but we are seeing artifacts
that concern me that 2 hrs may be insufficient.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 24
Date: Fri, 25 Sep 2009 09:29:12 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] cap survays
To: "Gonzales, Edith" <Edith.Gonzales <@t> providence.org>,
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC5552068537B4 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"

Edie,

What a challenge.
I would strongly recommend you give it a go.
I like to score fixation, processing and staining quality of slides I
and my staff produce.
It allows us to have control over the science of histotechnology not the
pathologists (they have enough to worry about - the diagnosis, clinical
demands etc).

When a department runs this way, it does not take long for the
pathologist to realise the invaluable resource their labs are. In fact
they become quite proud of it!

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Gonzales, Edith
Sent: Friday, 25 September 2009 5:50 AM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] cap survays

To all,

I would like to know what everyone is doing with the cap survay now that
it is scoring staining performance and not detremining a diagnosis.  Our
paths want us to score then and they not be involved.  Is this what
everone else is doing?

Edie

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------------------------------

Message: 25
Date: Fri, 25 Sep 2009 09:35:14 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: [Histonet] See you at the NSH
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC5552068537B5 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"

Hi all,

Just a note to say that I will be leaving next week for Birmingham
Alabama for the NSH conference.

It has taken me 30 years to get to a NSH and I am really looking forward
to it.

I hope to see you all (I will be the old, short, bald guy with the
Aussie accent)

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-

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Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

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------------------------------

Message: 26
Date: Thu, 24 Sep 2009 20:12:26 -0400
From: "Carrie Disbrow" <disbrc <@t> shands.ufl.edu>
Subject: [Histonet] Rhodanine Copper stain
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4ABBD2AA.72AC.0059.0 <@t> shands.ufl.edu>
Content-Type: text/plain; charset=US-ASCII

Hello!
I wonder if anyone knows if copper pigments can be stained in 3 micron FFPE human liver core biopsies using a Rhodanine Copper staining method? The procedure suggests 6- 8 microns. Our small amount of  tissue core biopsies are cut at 3 microns and if possible we would not like to cut the block again.
Thanks for your input.
Thank you,
Carrie Disbrow, HT ASCP  (soon to be BS, HTL ASCP :-))

------------------------------

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End of Histonet Digest, Vol 70, Issue 31
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