[Histonet] Separation Artifact

Kemlo Rogerson Kemlo.Rogerson <@t> waht.swest.nhs.uk
Tue Sep 22 02:59:50 CDT 2009

If its happening to an entire batch but not to other batches then you
can sorta rule out fixation unless you use different batches of fixative
for different batches of samples; logical yes? To put it another way if
you use the same fixative and fixation time on all batches but only one
is affected then it must be an issue with the machine. Not knowing which
one you are using makes this difficult but sometimes they go wrong or
there's the wrong fluid dispensed.

If however you are using different batches of fixatives on different
batches of samples you may have one batch of fixative that's incorrectly
made up. Hope that helps.

Kemlo Rogerson	
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
thecitan <@t> yahoo.com
Sent: 21 September 2009 21:27
To: Histonet
Subject: [Histonet] Separation Artifact

Hello histonet!

I'm trying to figure out the cause of some artifact in my ffpe h&e skin
slides. Every once and a while I get a batch of slides with this strange
separation artifact mostly around melanocytes. Its like there is a space
around each cell(s). Also the collagen seems to have some odd

I tried recuting to make sure there was no microtomy issues but the
artifact remains. I check my waterbath temp / soaked the blocks. Not
soaked the blocks. I'm at a loss. This happens to entire processing
batch. The doctors say they are still pathologically readable but I
still want to find a way to fix this.
Sent from my Verizon Wireless BlackBerry

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